概述

  • 产品名称Anti-PGAM1抗体
    参阅全部 PGAM1 一抗
  • 描述
    山羊多克隆抗体to PGAM1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Monkey
  • 免疫原

    Synthetic peptide: KAMEAVAAQGKAKK, corresponding to C terminal amino acids 241-254 of Human PGAM1.

  • 阳性对照
    • Human liver and brain lysates.
  • 常规说明GenBank Accession Number – NP_002620.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab2220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa.Can be blocked with Human PGAM1 peptide (ab22868).
IHC-P Use at an assay dependent concentration. PubMed: 21622897

靶标

Anti-PGAM1 antibody 图像

  • Anti-PGAM1 antibody (ab2220) at 0.01 µg/ml + Human liver lysate at 35 µg
    Developed using the ECL technique

    Primary incubated for 1 hour

  • ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab2220 staining PGAM1 in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    N, normal colonic mucosa; TII, Stage II; and TIV, Stage IV. Positive staining is indicated by red arrows.

Anti-PGAM1 antibody (ab2220)参考文献

This product has been referenced in:
  • Okuda J  et al. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice. PLoS One 8:e72173 (2013). WB ; Mouse . Read more (PubMed: 23951293) »
  • Hallows WC  et al. Regulation of Glycolytic Enzyme Phosphoglycerate Mutase-1 by Sirt1 Protein-mediated Deacetylation. J Biol Chem 287:3850-8 (2012). WB ; Human . Read more (PubMed: 22157007) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (BRAIN)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 20 µg
Specification BRAIN
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Nov 16 2015

Thank you for your enquiry. I have blasted the immunizing peptide sequence and found that 13 out of 14 amino acids were identical to the sequence of PGAM2. Therefore, it is likely that this product might cross react with PGAM2. However, since we hav...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"