Mouse, Rat, Cow
Recombinant fragment, corresponding to a region within amino acids 475-691 of Human PFKL (NP_001002021)
A431, A549, H1299, HeLa and HepG2 cell lines.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 90 kDa.
1/100 - 1/200.
PFKL is the Liver isozyme of PFK (Phosphofructokinase), which is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits.
PFK liver isoenzyme antibody
6 phosphofructokinase, liver type antibody
Western blot - Anti-PFKL antibody (ab97443)
Anti-PFKL antibody (ab97443) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg
Predicted band size: 90 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-PFKL antibody (ab97443)
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97443 at 1/200 dilution (upper panel) and merged with DNA probe (lower panel).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"