Anti-PFKFB4抗体(ab71622)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to PFKFB4
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human PFKFB4 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue and mouse brain tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab71622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
ICC/IF
  • 应用说明
    ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/100 - 1/500. Detects a band of approximately 60 kDa and many other bands (predicted molecular weight: 54 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    图片

    • Anti-PFKFB4 antibody (ab71622) at 1/100 dilution + mouse brain tissue lysate at 12.5 µg

      Predicted band size : 54 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71622 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by AEC staining.
    • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71622 at 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.
    • ICC/IF image of ab71622 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71622, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    文献

    ab71622 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from these antibodies.

    The details your customer has kindly provi...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116)
    Loading amount
    25 µg
    Specification
    HCT116
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 23 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NIH 3T3 cells)
    Loading amount
    25 µg
    Specification
    NIH 3T3 cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 23 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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