Anti-PFKFB1抗体(ab72590)

概述

  • 产品名称Anti-PFKFB1抗体
    参阅全部 PFKFB1 一抗
  • 描述
    兔多克隆抗体to PFKFB1
  • 特异性This antibody detects PFKFB1/2/4, but not PFKFB3.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human PFKFB1.

  • 阳性对照
    • Extracts from HeLa cells, HepG2 cells, COLO205 cells and 293 cells.

性能

应用

Our Abpromise guarantee covers the use of ab72590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/20000.

靶标

Anti-PFKFB1 antibody 图像

  • All lanes : Anti-PFKFB1 antibody (ab72590) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from COLO205 cells
    Lane 4 : Extracts from 293 cells
    Lane 5 : Extracts from 293 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 70 kDa,73 kDa. We are unsure as to the identity of these extra bands.

Anti-PFKFB1 antibody (ab72590)参考文献

ab72590 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply and for sending your WB image.

Perhaps that band is PFKFB4 isoform 3? That isoform is 413 a.a. long and is 47.6 kDa.

http://www.uniprot.org/blast/?about=Q6DTY7-3

I hope this information helps. Please ...

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Thank you for your inquiry.

This antibody hasn't been tested in mouse, so it is difficult to tell you for certain what the expected band pattern is. On top of that PFKFB1/2/4 have many isoforms with MW ranging from 25 - 52 kDa.

http:/...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 25 µg
Specification HCT116
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

提交于 Nov 23 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount 25 µg
Specification NIH 3T3 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"