概述

  • 产品名称
  • 描述
    兔多克隆抗体to PFKFB1
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from the center region of human PFKFB1 conjugated to KLH

  • 阳性对照
    • Jurkat cell line lysate and human hepatocarcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab71625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa).

靶标

Anti-PFKFB1 antibody 图像

  • Anti-PFKFB1 antibody (ab71625) at 1/240 dilution + Jurkat cell line lysate at 35 µg

    Predicted band size : 55 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab71625, at 1/50 dilution, staining PFKFB1 in formalin fixed, paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. ab71625 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

Anti-PFKFB1 antibody (ab71625)参考文献

This product has been referenced in:
  • Storm P  et al. Conserved features of cancer cells define their sensitivity to HAMLET-induced death; c-Myc and glycolysis. Oncogene 30:4765-79 (2011). WB . Read more (PubMed: 21643007) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
15 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing ('any Kd' Biorad precast)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Christoph Schmitt

Verified customer

提交于 Jul 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册