概述

  • 产品名称Anti-PFD1145c抗体
    参阅全部 PFD1145c 一抗
  • 描述
    小鼠多克隆抗体to PFD1145c
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Plasmodium falciparum
  • 免疫原

    Recombinant fusion protein containing a His tag:

    HPYDINNKNDDSYRYDISEE

    , corresponding to amino acids 240-259 of Plasmodium falciparum PFD1145c.

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性PFD1145c is a putative uncharacterized protein from Plasmodium falciparum>.
  • 数据库链接
    • 别名
      • Hypothetical protein PFD1145c antibody

    Anti-PFD1145c antibody 图像

    • All lanes : Anti-PFD1145c antibody (ab52699) at 1/1000 dilution

      Lane 1 : ~20µg of a total protein extract from E coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen
      Lane 2 : ~20µg of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag)

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Anti-PFD1145c antibody (ab52699)参考文献

    ab52699 has not yet been referenced specifically in any publications.

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