概述

  • 产品名称Anti-PF4抗体
    参阅全部 PF4 一抗
  • 描述
    兔多克隆抗体to PF4
  • 经测试应用适用于: WB, ELISA, Neutralising, IHC-P, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4)

性能

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应用

Our Abpromise guarantee covers the use of ab9561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hPF-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPF-4.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPF-4 (50 ng/ml), a concentration of 1.75 - 3.0 µg/ml of this antibody is required.
IHC-P 1/100.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to PF4 (Biotin) (ab83123).

Can be paired for Sandwich ELISA with ab83123

靶标

  • 功能Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰Binds non-covalently to a proteoglycan molecule.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-X-C motif chemokine 4 antibody
    • Chemokine (C X C motif) ligand 4 antibody
    • Chemokine (CXC motif) ligand 4 antibody
    • CXCL 4 antibody
    • CXCL4 antibody
    • Iroplact antibody
    • MGC138298 antibody
    • Oncostatin A antibody
    • Oncostatin-A antibody
    • OncostatinA antibody
    • PF 4 antibody
    • PF-4 antibody
    • PF4 antibody
    • Platelet factor 4 antibody
    • PLF4_HUMAN antibody
    • SCYB 4 antibody
    • SCYB4 antibody
    • short form antibody
    • Small inducible cytokine subfamily member 4 antibody
    see all

Anti-PF4 antibody 图像

  • Anti-PF4 antibody (ab9561) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
    Additional bands at : 18 kDa,30 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-PF4 antibody (ab9561)参考文献

This product has been referenced in:
  • Fritzer A  et al. Chemokine degradation by the Group A streptococcal serine proteinase ScpC can be reconstituted in vitro and requires two separate domains. Biochem J 422:533-42 (2009). WB . Read more (PubMed: 19552626) »
  • Houard X  et al. Mediators of neutrophil recruitment in human abdominal aortic aneurysms. Cardiovasc Res 82:532-41 (2009). IHC-P ; Human . Read more (PubMed: 19201759) »

See all 3 Publications for this product

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Ab9561 has been used in neutralizing assays and has been published byLambert MPet al.Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications.Blood110...

Read More
Application Western blot
Sample Mouse Tissue lysate - other (Platelet lysate)
Loading amount 50 µg
Specification Platelet lysate
Gel Running Conditions Reduced Denaturing (4-15% Tris Hcl gel)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Aug 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"