Anti-PEX5抗体[6E9] (ab125689)

概述

  • 产品名称Anti-PEX5抗体[6E9]
    参阅全部 PEX5 一抗
  • 描述
    小鼠单克隆抗体[6E9] to PEX5
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Dog, Human, Monkey
  • 免疫原

    Recombinant full length Human PEX5 produced in HEK293T cells (NP_000310).

  • 阳性对照
    • pCMV6-ENTRY PEX5-transfected HEK293T cells and lysate; HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, and MCF7 cell extracts; COS7 cells transiently transfected by pCMV6-ENTRY PEX5.
  • 常规说明Dilute in PBS (pH7.3) before use.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab125689 is purified from Mouse ascites fluid by affinity chromatography.
  • 克隆单克隆
  • 克隆编号6E9
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 71 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

靶标

  • 功能Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.
  • 组织特异性Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 疾病相关Defects in PEX5 are a cause of adrenoleukodystrophy neonatal (NALD) [MIM:202370]. NALD is a peroxisome biogenesis disorder (PBD) characterized by the accumulation of very long-chain fatty acids, adrenal insufficiency and mental retardation. Inheritance is autosomal recessive.
    Defects in PEX5 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life.
    Defects in PEX5 may be a cause of infantile Refsum disease (IRD) [MIM:266510]. IRD is a mild peroxisome biogenesis disorder (PBD). Clinical features include early onset, mental retardation, minor facial dysmorphism, retinopathy, sensorineural hearing deficit, hepatomegaly, osteoporosis, failure to thrive, and hypocholesterolemia. The biochemical abnormalities include accumulation of phytanic acid, very long chain fatty acids (VLCFA), di- and trihydroxycholestanoic acid and pipecolic acid.
  • 序列相似性Belongs to the peroxisomal targeting signal receptor family.
    Contains 7 TPR repeats.
  • 细胞定位Cytoplasm. Peroxisome membrane. Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane through a docking factor.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ50634 antibody
    • FLJ50721 antibody
    • FLJ51948 antibody
    • PBD2A antibody
    • PBD2B antibody
    • Peroxin 5 antibody
    • Peroxin-5 antibody
    • Peroxisomal biogenesis factor 5 antibody
    • Peroxisomal C terminal targeting signal import receptor antibody
    • Peroxisomal C-terminal targeting signal import receptor antibody
    • Peroxisomal targeting signal 1 (SKL type) receptor antibody
    • Peroxisomal targeting signal 1 receptor antibody
    • Peroxisomal targeting signal import receptor antibody
    • Peroxisomal targeting signal receptor 1 antibody
    • Peroxisome receptor 1 antibody
    • pex5 antibody
    • PEX5_HUMAN antibody
    • PTS1 BP antibody
    • PTS1 receptor antibody
    • PTS1-BP antibody
    • PTS1R antibody
    • PXR1 antibody
    see all

Anti-PEX5 antibody [6E9] 图像

  • All lanes : Anti-PEX5 antibody [6E9] (ab125689) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PEX5 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 71 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202062)
  • All lanes : Anti-PEX5 antibody [6E9] (ab125689) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 71 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202062)
  • ab125689 at 1/100 dilution staining PEX5 in COS7 cells transiently transfected with pCMV6-ENTRY PEX5 by Immunofluorescence.
  • ab125689 at 1/100 dilution staining PEX5 in HEK293T cells transfected with either pCMV6-ENTRY PEX5 (Red) or empty vector control plasmid (Blue) by Flow Cytometry.

Anti-PEX5 antibody [6E9] (ab125689)参考文献

ab125689 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab125689.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"