Pescadillo overexpression 293T裂解液(全细胞) (ab94282)

概述

  • 产品名称Pescadillo overexpression 293T裂解液(全细胞)
    参阅全部 Pescadillo 细胞裂解液
  • 描述
    Pescadillo overexpression裂解液
  • 常规说明ab94282 is a 293T cell transfected lysate in which Human Pescadillo has been transiently over-expressed using a pCMV-Pescadillo plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Tissue specificity: Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin. Similarity: Belongs to the pescadillo family. Contains 1 BRCT domain. PTM: Sumoylated.

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应用

Our Abpromise guarantee covers the use of ab94282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Pescadillo overexpression 293T lysate (whole cell) 图像

  • ab94282 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Pescadillo antibody (ab88543) at 1/500 dilution

    Lane 1 : Pescadillo overexpression 293T lysate (whole cell) (ab94282)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Pescadillo overexpression 293T lysate (whole cell) (ab94282)参考文献

ab94282 has not yet been referenced specifically in any publications.

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