概述

  • 产品名称
    Anti-Pescadillo抗体
    参阅全部 Pescadillo 一抗
  • 描述
    兔多克隆抗体to Pescadillo
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo (NP_055118.1)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab72539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
  • 组织特异性
    Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin.
  • 序列相似性
    Belongs to the pescadillo family.
    Contains 1 BRCT domain.
  • 翻译后修饰
    Sumoylated.
  • 细胞定位
    Nucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.
  • Information by UniProt
  • 数据库链接
    • 别名
      • FLJ43790 antibody
      • Homo sapiens pescadillo mRNA complete cds antibody
      • PES antibody
      • PES 1 antibody
      • pes1 antibody
      • PESC_HUMAN antibody
      • Pescadillo (zebrafish) homolog 1 containing BRCT domain antibody
      • Pescadillo homolog 1 antibody
      • Pescadillo homolog 1 containing BRCT domain (zebrafish) antibody
      • Pescadillo homolog antibody
      • Pescadillo ribosomal biogenesis factor 1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pescadillo antibody (ab72539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pescadillo antibody (ab72539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Pescadillo with ab72539 at 1/200 (1µg/ml). Detection: DAB.
    • Western blot - Pescadillo antibody (ab72539)
      Western blot - Pescadillo antibody (ab72539)
      All lanes : Anti-Pescadillo antibody (ab72539) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 68 kDa
      Observed band size: 68 kDa
      Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.

    • Immunoprecipitation - Pescadillo antibody (ab72539)
      Immunoprecipitation - Pescadillo antibody (ab72539)
      Detection of Human Pescadillo by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72539 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

    文献

    ab72539 has not yet been referenced specifically in any publications.

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