概述
- 产品名称Anti-Pescadillo抗体
参阅全部 Pescadillo 一抗 - 描述兔多克隆抗体to Pescadillo
- 经测试应用适用于: IHC-P, WB, IPmore details
- 种属反应性与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- 免疫原
Synthetic peptide corresponding to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo (NP_055118.1)
- 阳性对照
- HeLa and 293T whole cell lysates.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab72539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
靶标
- 功能Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
- 组织特异性Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin.
- 序列相似性Belongs to the pescadillo family.
Contains 1 BRCT domain. - 翻译后修饰Sumoylated.
- 细胞定位Nucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.
- Information by UniProt
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数据库链接
- Entrez Gene: 23481 Human
- Omim: 605819 Human
- SwissProt: O00541 Human
- Unigene: 517543 Human
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别名
- FLJ43790 antibody
- Homo sapiens pescadillo mRNA complete cds antibody
- PES antibody
see all
Anti-Pescadillo antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pescadillo antibody (ab72539)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Pescadillo with ab72539 at 1/200 (1µg/ml). Detection: DAB. -
Western blot - Pescadillo antibody (ab72539)All lanes : Anti-Pescadillo antibody (ab72539) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 68 kDa
Observed band size : 68 kDa
Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Pescadillo antibody (ab72539)Detection of Human Pescadillo by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72539 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Anti-Pescadillo antibody (ab72539)参考文献
ab72539 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"