Anti-Peroxiredoxin-SO3抗体(ab16830)

概述

  • 产品名称
    Anti-Peroxiredoxin-SO3抗体
    参阅全部 Peroxiredoxin-SO3 一抗
  • 描述
    兔多克隆抗体to Peroxiredoxin-SO3
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic sulfonylated peptide (Human) conjugated to KLH, corresponding to Prx I-IV active site.

  • 阳性对照
    • H2O2 treated HeLa whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab16830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 22 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 18826942

靶标

  • 相关性
    Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
  • 别名
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin 2 antibody
    • Peroxiredoxin 3 antibody
    • Peroxiredoxin 4 antibody
    • PRDX1 antibody
    • PRDX2 antibody
    • PRDX3 antibody
    • PRDX4 antibody
    see all

图片



  • Predicted band size : 22 kDa

    Upper blot - Prx-SO3 antibody (ab16830) at 1/2000
    Lower blot - Prx1 antibody as a loading control

    Lane 1: H2O2 untreated HeLa cell lysate
    Lane 2: H2O2 treated HeLa cell lysate
    Lane 3: Reduced recombinant Prx1
    Lane 4: Oxidized recombinant Prx1

  • IHC image of ab16830 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16830,1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • Magarin M  et al. Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress. J Mol Cell Cardiol 97:1-14 (2016). Read more (PubMed: 27106802) »
  • Navarro-Yepes J  et al. Inhibition of Protein Ubiquitination by Paraquat and 1-Methyl-4-Phenylpyridinium Impairs Ubiquitin-Dependent Protein Degradation Pathways. Mol Neurobiol 53:5229-51 (2016). Read more (PubMed: 26409479) »

See all 32 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Rat Tissue lysate - whole (Aorta)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
50 µg
Specification
Aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. D Richard

Verified customer

提交于 Jul 27 2015

Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (TCA prep)
Gel Running Conditions
Reduced Denaturing (4 -12% NuPAGE bis-tris gel with MES buffer and antioxidant)
Loading amount
10 µg
Treatment
With or without 1mM H2O2 for 15 minutes
Specification
TCA prep
Blocking step
0.5% BSA and 0.5% milk in TBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Helen Causton

Verified customer

提交于 Apr 22 2015

Application
IHC - Wholemount
Sample
Rat Tissue (Mesenteric Venule)
Specification
Mesenteric Venule
Username

Abcam user community

Verified customer

提交于 Dec 18 2012

Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage ...

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Thank you for contacting Abcam and I am sorry for the late response.
I have talked to the lab about the protocol that was used to oxidize the PrX1 protein (ab16830) and they recommended fallowing the protocol in the attached reference.
Please...

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Thank you for contacting us with your questions. We have only tested these antibodies under reducing conditions in WB. They may be suitable for use under non-reducing conditions as well, but this has not been validated in-house. I hope this information...

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Thank you for contacting us.  We have only tested these Peroxiredoxin antibodies under reducing conditions in WB.  They may be suitable for use under non-reducing conditions as well, but this has not been validated in house.  The unconjugated secondary...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Loading amount
30 µg
Specification
Mouse Embryonic Fibroblasts
Treatment
100 uM H2O2 for 6 hrs
Gel Running Conditions
Non-reduced Denaturing (10% SDS)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary neurons)
Loading amount
600000 cells
Specification
primary neurons
Treatment
H2O2 100uM 1h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Dec 21 2006

Thank you for your enquiry. I can confirm that milk was used as a blocking agent to test ab16830 by western blotting. Please let me know if you have any further inquiries.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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