重组Anti-Peroxiredoxin 6抗体[EPR3755] (ab92322)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3755] to Peroxiredoxin 6
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Peroxiredoxin 6抗体[EPR3755]
参阅全部 Peroxiredoxin 6 一抗 -
描述
兔单克隆抗体[EPR3755] to Peroxiredoxin 6 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Peroxiredoxin 6 aa 200-300. The exact sequence is proprietary.
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阳性对照
- HeLa, MCF7, HepG2, HAP1 and A549 cell lysates, Human brain tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3755 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
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说明 |
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IP
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa. |
靶标
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功能
Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. -
序列相似性
Belongs to the ahpC/TSA family. Rehydrin subfamily.
Contains 1 thioredoxin domain. -
细胞定位
Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles. - Information by UniProt
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数据库链接
- Entrez Gene: 9588 Human
- Entrez Gene: 11758 Mouse
- Entrez Gene: 94167 Rat
- Omim: 602316 Human
- SwissProt: P30041 Human
- SwissProt: O08709 Mouse
- SwissProt: O35244 Rat
- Unigene: 120 Human
see all -
别名
- 1 Cys antibody
- 1 Cys peroxiredoxin antibody
- 1 Cys PRX antibody
see all
图片
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All lanes : Anti-Peroxiredoxin 6 antibody [EPR3755] (ab92322) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : PRDX6 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25 kDaab92322 was shown to react with PRDX6 in wild-type HAP1 cells in Western blot with loss of signal observed in a PRDX6 knockout cell line. Wild-type HAP1 and PRDX6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab92322 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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Immunoprecipitation of PRDX6 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 2.0μg of ab92322 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab133348 at 1/1000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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All lanes : Anti-Peroxiredoxin 6 antibody [EPR3755] (ab92322) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab92322 被引用在 2 文献中.
- Buonora JE et al. Multivariate analysis of traumatic brain injury: development of an assessment score. Front Neurol 6:68 (2015). PubMed: 25870583
- Pütz SM et al. Malignant transformation in a defined genetic background: proteome changes displayed by 2D-PAGE. Mol Cancer 9:254 (2010). WB ; Human . PubMed: 20860785