Anti-Peroxiredoxin 6抗体(ab73350)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-Peroxiredoxin 6抗体
参阅全部 Peroxiredoxin 6 一抗 -
描述兔多克隆抗体to Peroxiredoxin 6
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经测试应用适用于: IHC-P, WB, ICC/IF, IPmore details
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种属反应性与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Pig, Xenopus laevis, Non human primates
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Peroxiredoxin 6.
(Peptide available as ab73681.)
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阳性对照
- This antibody gave a positive signal in the following lysates: Testis (Rat) Tissue, Lung (Rat) Tissue, Liver (Mouse) Tissue, Ramos Whole Cell
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab73350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IP | Use a concentration of 5 µg/ml. |
靶标
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功能Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
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序列相似性Belongs to the ahpC/TSA family. Rehydrin subfamily.
Contains 1 thioredoxin domain. -
细胞定位Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
- Information by UniProt
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数据库链接
- Entrez Gene: 9588 Human
- Entrez Gene: 11758 Mouse
- Entrez Gene: 399538 Pig
- Entrez Gene: 94167 Rat
- Omim: 602316 Human
- SwissProt: P30041 Human
- SwissProt: O08709 Mouse
- SwissProt: Q9TSX9 Pig
see all -
别名
- 1 Cys antibody
- 1 Cys peroxiredoxin antibody
- 1 Cys PRX antibody
see all
Anti-Peroxiredoxin 6 antibody 图像
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Western blot - Peroxiredoxin 6 antibody (ab73350)All lanes : Anti-Peroxiredoxin 6 antibody (ab73350) at 1 µg/ml
Lane 1 : Testis (Rat) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 25 kDa
Observed band size : 25 kDa
Additional bands at : 48 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes -
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 6 antibody (ab73350)ICC/IF image of ab73350 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (10 min) HeLa, HepG2, Hek293 and MCF7 cells at 1µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 6 antibody (ab73350)IHC image of Peroxiredoxin 6 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73350, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX -
Western blot - Anti-Peroxiredoxin 6 antibody (ab73350) -
Immunoprecipitation - Anti-Peroxiredoxin 6 antibody (ab73350)Peroxiredoxin 6 was immunoprecipitated using 0.5mg Rat Testis tissue lysate, 5µg of Rabbit polyclonal to Peroxiredoxin 6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Rat Testis tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73350.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 25KDa: Peroxiredoxin 6
实验方案
Anti-Peroxiredoxin 6 antibody (ab73350)参考文献
This product has been referenced in:
- Wiggins LM Morphological changes and altered expression of antioxidant proteins in a heterozygous dynein mutant; a mouse model of spinal muscular atrophy. Oxid Antioxid Med Sci 3:161-173 (0). Read more (PubMed: 25866698) »
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