概述

  • 产品名称
    Anti-Peroxiredoxin 6抗体
    参阅全部 Peroxiredoxin 6 一抗
  • 描述
    兔多克隆抗体to Peroxiredoxin 6
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Pig, Xenopus laevis, Non human primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Peroxiredoxin 6.

    参阅Abcam的专有抗源政策

    (Peptide available as ab73681.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Testis (Rat) Tissue, Lung (Rat) Tissue, Liver (Mouse) Tissue, Ramos Whole Cell

性能

应用

Our Abpromise guarantee covers the use of ab73350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.

靶标

  • 功能
    Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
  • 序列相似性
    Belongs to the ahpC/TSA family. Rehydrin subfamily.
    Contains 1 thioredoxin domain.
  • 细胞定位
    Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • 1 Cys antibody
    • 1 Cys peroxiredoxin antibody
    • 1 Cys PRX antibody
    • 1 cysPrx antibody
    • 1-Cys peroxiredoxin antibody
    • 1-Cys PRX antibody
    • 24 kDa protein antibody
    • 9430088D19Rik antibody
    • AA690119 antibody
    • Acidic calcium independent phospholipase A2 antibody
    • Acidic calcium-independent phospholipase A2 antibody
    • aiPLA2 antibody
    • Antioxidant protein 2 antibody
    • AOP2 antibody
    • Aop2 rs3 antibody
    • Brp 12 antibody
    • Ciliary body glutathione peroxidase antibody
    • CP 3 antibody
    • EC 1.11.1.15 antibody
    • EC 1.11.1.7 antibody
    • EC 3.1.1. antibody
    • Epididymis secretory sperm binding protein Li 128m antibody
    • GPx antibody
    • HEL S 128m antibody
    • KIAA0106 antibody
    • Liver 2D page spot 40 antibody
    • Ltw4 antibody
    • Lvtw 4 antibody
    • MGC46173 antibody
    • mKIAA0106 antibody
    • Non selenium glutathione peroxidase antibody
    • Non-selenium glutathione peroxidase antibody
    • NSGPx antibody
    • ORF06 antibody
    • OTTHUMP00000032693 antibody
    • p29 antibody
    • Peroxiredoxin-6 antibody
    • Peroxiredoxin6 antibody
    • PHGPx antibody
    • Phospholipase A2 lysosomal antibody
    • PLA2 antibody
    • PRDX 6 antibody
    • Prdx5 antibody
    • PRDX6 antibody
    • Prdx6 rs3 antibody
    • PRDX6_HUMAN antibody
    • PRX antibody
    • Red blood cells page spot 12 antibody
    • Thiol specific antioxidant protein antibody
    see all

图片

  • Western blot - Peroxiredoxin 6 antibody (ab73350)
    Western blot - Peroxiredoxin 6 antibody (ab73350)
    All lanes : Anti-Peroxiredoxin 6 antibody (ab73350) at 1 µg/ml

    Lane 1 : Testis (Rat) Tissue Lysate
    Lane 2 : Lung (Rat) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa
    Additional bands at: 48 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes
  • Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 6 antibody (ab73350)
    Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 6 antibody (ab73350)
    ICC/IF image of ab73350 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (10 min) HeLa, HepG2, Hek293 and MCF7 cells at 1µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 6 antibody (ab73350)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 6 antibody (ab73350)
    IHC image of Peroxiredoxin 6 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73350, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • Western blot - Anti-Peroxiredoxin 6 antibody (ab73350)
    Western blot - Anti-Peroxiredoxin 6 antibody (ab73350)
    Anti-Peroxiredoxin 6 antibody (ab73350) at 1 µg/ml + Recombinant human Peroxiredoxin 6 protein (ab87631) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-Peroxiredoxin 6 antibody (ab73350)
    Immunoprecipitation - Anti-Peroxiredoxin 6 antibody (ab73350)
    Peroxiredoxin 6 was immunoprecipitated using 0.5mg Rat Testis tissue lysate, 5µg of Rabbit polyclonal to Peroxiredoxin 6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Testis tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73350.
    Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
    Band: 25KDa: Peroxiredoxin 6

文献

This product has been referenced in:
  • Wiggins LM Morphological changes and altered expression of antioxidant proteins in a heterozygous dynein mutant; a mouse model of spinal muscular atrophy. Oxid Antioxid Med Sci 3:161-173 (0). Read more (PubMed: 25866698) »

See 1 Publication for this product

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