Anti-Peroxiredoxin 6抗体(ab59543)
Key features and details
- Rabbit polyclonal to Peroxiredoxin 6
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Peroxiredoxin 6抗体
参阅全部 Peroxiredoxin 6 一抗 -
描述
兔多克隆抗体to Peroxiredoxin 6 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Rat Peroxiredoxin 6.
Database link: P30041 -
阳性对照
- ICC: Mouse macrophages, HeLa cells. IHC-P: Astrocytes.
-
常规说明
Staining pattern: cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. Stains human brain astrocytes in Parkinson's and Alzheimer's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading...
-
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59543于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
ICC/IF | (1) |
1/50 - 1/100.
|
说明 |
---|
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/50 - 1/100. |
靶标
-
功能
Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. -
序列相似性
Belongs to the ahpC/TSA family. Rehydrin subfamily.
Contains 1 thioredoxin domain. -
细胞定位
Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles. - Information by UniProt
-
数据库链接
- Entrez Gene: 9588 Human
- Omim: 602316 Human
- SwissProt: P30041 Human
- Unigene: 120 Human
-
别名
- 1 Cys antibody
- 1 Cys peroxiredoxin antibody
- 1 Cys PRX antibody
see all
图片
-
ab59543 staining Peroxiredoxin 6 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.
-
ab59543 at 1/100 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by Immunohistochemistry. Secondary antibody anti-rabbit IgG conjugated to Cy3 (1/100).
-
ab59543 at 1/1000 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by IHC-P. Secondary antibody Donkey anti-rabbit conjugated to biotin.
-
ICC/IF image of ab59543 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59543, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (31)
ab59543 被引用在 31 文献中.
- Beltrán-García J et al. Extracellular Histones Activate Endothelial NLRP3 Inflammasome and are Associated with a Severe Sepsis Phenotype. J Inflamm Res 15:4217-4238 (2022). PubMed: 35915852
- Di Sanzo M et al. Ferritin Heavy Chain Binds Peroxiredoxin 6 and Inhibits Cell Proliferation and Migration. Int J Mol Sci 23:N/A (2022). PubMed: 36361777
- Queiroz RF et al. Hydrogen peroxide signaling via its transformation to a stereospecific alkyl hydroperoxide that escapes reductive inactivation. Nat Commun 12:6626 (2021). PubMed: 34785665
- Naguib S et al. Retinal oxidative stress activates the NRF2/ARE pathway: An early endogenous protective response to ocular hypertension. Redox Biol 42:101883 (2021). PubMed: 33579667
- Esparza-Moltó PB et al. Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition. PLoS Biol 19:e3001252 (2021). PubMed: 33983919