兔多克隆抗体to Peroxiredoxin 5
Mouse, Rat, Horse, Cat, Dog, Pig, African green monkey
Synthetic peptide corresponding to a region within the internal sequence amino acids 71-120 (TDLLLDDSLV SIFGNRRLKR FSMVVQDGIV KALNVEPDGT GLTCSLAPNI) of Human Peroxiredoxin 5 (NP_857635).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately <22 kDa (predicted molecular weight: 22 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/12500.
Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
Belongs to the peroxiredoxin 2 family.
Contains 1 thioredoxin domain.
Mitochondrion. Cytoplasm. Peroxisome.
Information by UniProt
- ACR1 antibody
- Alu co repressor 1 antibody
- Alu corepressor 1 antibody
Western blot - Anti-Peroxiredoxin 5 antibody (ab83045)
Anti-Peroxiredoxin 5 antibody (ab83045) at 1 µg/ml (5% skim milk / PBS buffer) + fetal lung lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
22 kDaObserved band size:
<22 kDa (why is the actual band size different from the predicted?
Gel concentration 12%
has not yet been referenced specifically in any publications.
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