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Our Abpromise guarantee covers the use of ab16942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 24 kDa.|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: A549 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab16942 observed at 17 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab16942 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab16942 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
This image is courtesy of an anonymous Abreview
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human lung, respiratory epithelium tissue labeling Peroxiredoxin with ab16942.
ab16942 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"