使用敲除细胞株进行验证

Anti-Peroxiredoxin 5抗体[12A] (ab16942)

概述

  • 产品名称Anti-Peroxiredoxin 5抗体[12A]
    参阅全部 Peroxiredoxin 5 一抗
  • 描述
    小鼠单克隆抗体[12A] to Peroxiredoxin 5
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant human fragment protein (without mitochondrial leader sequence) purified from E.coli.

  • 阳性对照
    • HeLa cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.03% Sodium azide
    Constituents: HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号12A
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 24 kDa.

靶标

  • 功能Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the peroxiredoxin 2 family.
    Contains 1 thioredoxin domain.
  • 细胞定位Mitochondrion. Cytoplasm. Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACR1 antibody
    • Alu co repressor 1 antibody
    • Alu corepressor 1 antibody
    • Antioxidant enzyme B166 antibody
    • AOEB166 antibody
    • B166 antibody
    • epididymis secretory protein Li 55 antibody
    • HEL-S-55 antibody
    • Liver tissue 2D-page spot 71B antibody
    • mitochondrial antibody
    • Peroxiredoxin 5, mitochondrial antibody
    • Peroxiredoxin V antibody
    • Peroxiredoxin-5 antibody
    • Peroxisomal antioxidant enzyme antibody
    • PLP antibody
    • PMP20 antibody
    • PRDX 5 antibody
    • PRDX5 antibody
    • PRDX5_HUMAN antibody
    • PRDX6 antibody
    • Prx-V antibody
    • PRXV antibody
    • SBBI10 antibody
    • Thioredoxin peroxidase PMP20 antibody
    • Thioredoxin reductase antibody
    • TPx type VI antibody
    see all

Anti-Peroxiredoxin 5 antibody [12A] 图像



  • Predicted band size : 24 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab16942 observed at 17 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab16942 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab16942 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-Peroxiredoxin 5 antibody [12A] (ab16942) at 1/5000 dilution + Rat cerebellum whole tissue lysate at 30 µg

    Secondary
    Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa


    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview

    Secondary antibody - anti-mouse HRP (ab97046)

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human lung, respiratory epithelium tissue labeling Peroxiredoxin with ab16942.

Anti-Peroxiredoxin 5 antibody [12A] (ab16942)参考文献

ab16942 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Rat Tissue lysate - whole (cerebellum)
Specification cerebellum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Mar 17 2014

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