Anti-Peroxiredoxin 4抗体(ab95232)


  • 产品名称Anti-Peroxiredoxin 4抗体
    参阅全部 Peroxiredoxin 4 一抗
  • 描述
    兔多克隆抗体to Peroxiredoxin 4
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Peroxiredoxin 4.

  • 阳性对照
    • This antibody gave a positive signal in the following human whole cell lysates: HeLa; Jurkat; HEK293; Raji; HepG2.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab95232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • 功能Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
  • 序列相似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 细胞定位Cytoplasm. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antioxidant enzyme 372 antibody
    • Antioxidant enzyme AOE372 antibody
    • AOE37 2 antibody
    • AOE37-2 antibody
    • AOE372 antibody
    • EC antibody
    • Peroxiredoxin IV antibody
    • Peroxiredoxin-4 antibody
    • Peroxiredoxin4 antibody
    • PRDX 4 antibody
    • Prdx4 antibody
    • PRDX4_HUMAN antibody
    • PRX 4 antibody
    • Prx IV antibody
    • Prx-IV antibody
    • PRX4 antibody
    • PrxIV antibody
    • Thioredoxin dependent peroxide reductase A0372 antibody
    • Thioredoxin Peroxidase (Antioxidant Enzyme) antibody
    • Thioredoxin peroxidase antibody
    • Thioredoxin peroxidase AO372 antibody
    • Thioredoxin-dependent peroxide reductase A0372 antibody
    • TRANK antibody
    see all

Anti-Peroxiredoxin 4 antibody 图像

  • All lanes : Anti-Peroxiredoxin 4 antibody (ab95232) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,70 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab95232 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85232, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and MCF7 cells at 5µg/ml.

Anti-Peroxiredoxin 4 antibody (ab95232)参考文献

ab95232 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95232.
Please use the links above to contact us or submit feedback about this product.