产品名称 Anti-Peroxiredoxin 4抗体 参阅全部 Peroxiredoxin 4 一抗
兔多克隆抗体to Peroxiredoxin 4
WB, ICC/IF more details
Mouse, Rat, Cow
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Peroxiredoxin 4.
This antibody gave a positive signal in the following human whole cell lysates: HeLa; Jurkat; HEK293; Raji; HepG2.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 31 kDa).
Use a concentration of 5 µg/ml.
功能 Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
序列相似性 Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain.
细胞定位 Cytoplasm. Secreted.
Information by UniProt
Antioxidant enzyme 372 antibody
Antioxidant enzyme AOE372 antibody
AOE37 2 antibody
Anti-Peroxiredoxin 4 antibody 图像
Western blot - Peroxiredoxin 4 antibody (ab95232)
All lanes : Anti-Peroxiredoxin 4 antibody (ab95232) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 31 kDa Observed band size : 27 kDa ( why is the actual band size different from the predicted?) Additional bands at : 20 kDa,70 kDa,80 kDa. We are unsure as to the identity of these extra bands. Exposure time : 30 seconds
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 4 antibody (ab95232)
ICC/IF image of ab95232 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ( ab85232, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and MCF7 cells at 5µg/ml.
Anti-Peroxiredoxin 4 antibody (ab95232)参考文献
has not yet been referenced specifically in any publications.
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