概述

  • 产品名称Anti-Peroxiredoxin 4抗体
    参阅全部 Peroxiredoxin 4 一抗
  • 描述
    兔多克隆抗体to Peroxiredoxin 4
  • 特异性ab59542 reacts with Peroxiredoxin 4.
  • 经测试应用适用于: WB, ELISA, IHC-Fr, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human Peroxiredoxin 4 aa 65-83.
    Sequence:

    SRVSVADHSLHLSKAKISK

  • 阳性对照
    • Non-ciliated bronchiolar cells (Clara cells) in rat lung and sebaceous glands in rat skin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/4000. Predicted molecular weight: 31 kDa.
ELISA 1/1000 - 1/4000.
IHC-Fr 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
IP Use at an assay dependent concentration.

靶标

  • 功能Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
  • 序列相似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 细胞定位Cytoplasm. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antioxidant enzyme 372 antibody
    • Antioxidant enzyme AOE372 antibody
    • AOE37 2 antibody
    • AOE37-2 antibody
    • AOE372 antibody
    • EC 1.11.1.15 antibody
    • Peroxiredoxin IV antibody
    • Peroxiredoxin-4 antibody
    • Peroxiredoxin4 antibody
    • PRDX 4 antibody
    • Prdx4 antibody
    • PRDX4_HUMAN antibody
    • PRX 4 antibody
    • Prx IV antibody
    • Prx-IV antibody
    • PRX4 antibody
    • PrxIV antibody
    • Thioredoxin dependent peroxide reductase A0372 antibody
    • Thioredoxin Peroxidase (Antioxidant Enzyme) antibody
    • Thioredoxin peroxidase antibody
    • Thioredoxin peroxidase AO372 antibody
    • Thioredoxin-dependent peroxide reductase A0372 antibody
    • TRANK antibody
    see all

Anti-Peroxiredoxin 4 antibody 图像

  • Peroxiredoxin 4 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µl of Rabbit polyclonal to Peroxiredoxin 4 sera and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59542.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 27kDa; Peroxiredoxin 4, non specific - as present in control (lane 2); 27kDa: We are confident this was due to slight lane contamination and the band seen in the IP lane is our target of interest.

  • ab59542 at 1/100 dilution staining Peroxiredoxin 4 in sebaceous glands in rat skin by Immunohistochemistry. Secondary antibody Jackson biotinylated Donkey anti-rabbit (1/2000) followed by Vector ABC kit and visualised with DAB substrate.
  • ab59542 at 1/100 dilution staining Peroxiredoxin 4 in Non-ciliated bronchiolar cells (Clara cells) in rat lung by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
  • All lanes : Anti-Peroxiredoxin 4 antibody (ab59542) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 74 kDa,98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
    The band observed at 27 kDa could potentially be a cleaved form of Peroxiredoxin 4 due to the presence of a 39 amino acid signal peptide.

Anti-Peroxiredoxin 4 antibody (ab59542)参考文献

This product has been referenced in:
  • Jiang H  et al. Expression of peroxiredoxin 1 and 4 promotes human lung cancer malignancy. Am J Cancer Res 4:445-60 (2014). WB ; Human . Read more (PubMed: 25232487) »
  • Meng Y  et al. Downregulated expression of peroxiredoxin 4 in granulosa cells from polycystic ovary syndrome. PLoS One 8:e76460 (2013). WB, IHC-P ; Human . Read more (PubMed: 24098506) »

See all 8 Publications for this product

Product Wall

Thank you for contacting us.

The source of ab16943 informed us that theimmunogen used for this antibody isa recombinant human fragment protein (without secretion leader sequence) purified from E.coli. Only the region “WETEERPRTREEECHFYAG...

Read More

I received some useful information from the laboratory that developed this antibody: "In human lung we see two very clean bands, one around 29kD and the other around 60kD. This protein is reported as being synthesised as a 31kD protein and a signal...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"