Anti-Peroxiredoxin 4抗体[7A1] (ab16943)

概述

  • 产品名称Anti-Peroxiredoxin 4抗体[7A1]
    参阅全部 Peroxiredoxin 4 一抗
  • 描述
    小鼠单克隆抗体[7A1] to Peroxiredoxin 4
  • 经测试应用适用于: ELISA, WB, IP, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Ferret
  • 免疫原

    Recombinant human fragment (without secretion leader sequence) purified from E.coli.

  • 阳性对照
    • Hela cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.03% Sodium Azide
    Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号7A1
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 29 kDa (predicted molecular weight: 31 kDa).
IP Use a concentration of 1 - 2 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
  • 序列相似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 细胞定位Cytoplasm. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antioxidant enzyme 372 antibody
    • Antioxidant enzyme AOE372 antibody
    • AOE37 2 antibody
    • AOE37-2 antibody
    • AOE372 antibody
    • EC 1.11.1.15 antibody
    • Peroxiredoxin IV antibody
    • Peroxiredoxin-4 antibody
    • Peroxiredoxin4 antibody
    • PRDX 4 antibody
    • Prdx4 antibody
    • PRDX4_HUMAN antibody
    • PRX 4 antibody
    • Prx IV antibody
    • Prx-IV antibody
    • PRX4 antibody
    • PrxIV antibody
    • Thioredoxin dependent peroxide reductase A0372 antibody
    • Thioredoxin Peroxidase (Antioxidant Enzyme) antibody
    • Thioredoxin peroxidase antibody
    • Thioredoxin peroxidase AO372 antibody
    • Thioredoxin-dependent peroxide reductase A0372 antibody
    • TRANK antibody
    see all

Anti-Peroxiredoxin 4 antibody [7A1] 图像



  • Predicted band size : 31 kDa
    Ab16943 staining Peroxiredoxin 4 in cell lysates by Western blot. Lanes 1, 2 and 3 represent Hela cell lysates, 293T cell lysates and Jurkat cell lysates respectively. Ab16943 staining Peroxiredoxin 4 in cell lysates by Western blot. Lanes 1, 2 and 3 represent Hela cell lysates, 293T cell lysates and Jurkat cell lysates respectively.
  • ICC/IF image of ab16943 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab16943 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16943, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-Peroxiredoxin 4 antibody [7A1] (ab16943)参考文献

This product has been referenced in:
  • Cao Z  et al. Lack of an efficient endoplasmic reticulum-localized recycling system protects peroxiredoxin IV from hyperoxidation. J Biol Chem 289:5490-8 (2014). WB ; Human . Read more (PubMed: 24403061) »
  • McDonagh B  et al. Differential cysteine labeling and global label-free proteomics reveals an altered metabolic state in skeletal muscle aging. J Proteome Res 13:5008-21 (2014). WB ; Mouse . Read more (PubMed: 25181601) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 10 2016

Application Western blot
Loading amount 120 µg
Gel Running Conditions Reduced Denaturing (15)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 16 2014

Thank you very much for confirming the details.

I have created a free of charge replacement order for you. The order number is 1219156 which will be dispatched on Monday.

Please let me know if you need further assistance. I hope the F...

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Thank you for your email. My colleague Kate is away so I have taken over the case.

The only question I would like to ask is, was the antibody in liquid state at -20C?; it should be as it contains 50% Glycerol at 1:1 ratio and freezing point of...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody once it was frozen.

I would like to reassure you that this antibody is tested and covered by our 6 mont...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle (AT))
Loading amount 50 µg
Specification Skeletal muscle (AT)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 Sep 28 2012

Thank you for your reply.

If you can determine from your experimentsthe region where the antibody binds,Iwould encourage you to submit an Abreview via the online product datasheet with your results. We always appreciate customer feedback and w...

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Thank you for contacting us.

The source of ab16943 informed us that theimmunogen used for this antibody isa recombinant human fragment protein (without secretion leader sequence) purified from E.coli. Only the region “WETEERPRTREEECHFYAG...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Bonemarrow)
Specification Bonemarrow
Fixative Paraformaldehyde
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Apr 25 2012

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