概述

  • 产品名称Anti-Peroxiredoxin 3抗体[4G10]
    参阅全部 Peroxiredoxin 3 一抗
  • 描述
    小鼠单克隆抗体[4G10] to Peroxiredoxin 3
  • 经测试应用适用于: IHC-P, WB, IP, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Recombinant human protein, corresponding to amino acids 63-256 of Peroxiredoxin 3, purified from E.coli

  • 阳性对照
    • HeLa cell lysates

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab16753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 21 kDa). This corresponds with the mature form of the protein.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 18826942

靶标

  • 功能Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
  • 序列相似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antioxidant protein 1 antibody
    • AOP 1 antibody
    • AOP-1 antibody
    • AOP1 antibody
    • HBC189 antibody
    • MER5 antibody
    • MGC104387 antibody
    • MGC24293 antibody
    • mitochondrial antibody
    • peroxiredoxin 3 antibody
    • Peroxiredoxin III antibody
    • Peroxiredoxin-3 antibody
    • PRDX3 antibody
    • PRDX3_HUMAN antibody
    • PRO1748 antibody
    • Protein MER5 homolog antibody
    • PRX III antibody
    • Prx-III antibody
    • PRX3 antibody
    • SP 22 antibody
    • SP-22 antibody
    • SP22 antibody
    • Thioredoxin dependent peroxide reductase mitochondrial antibody
    • Thioredoxin-dependent peroxide reductase antibody
    see all

Anti-Peroxiredoxin 3 antibody [4G10] 图像



  • Predicted band size : 21 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Peroxiredoxin 3 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab16753 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab16753 was shown to specifically react with Peroxiredoxin 3 when Peroxiredoxin 3 knockout samples were used. Wild-type and Peroxiredoxin 3 knockout samples were subjected to SDS-PAGE. ab16753 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.



  • Predicted band size : 21 kDa

    Western Blot analysis of cell lysates (20µg) using ab16753 at 1/1000.

    Lane 1: HeLa cell lysates
    Lane 2: 293T cell lysates
    Lane 3: Jurkat cell lysates

    Secondary antibody conjugated to HRP

    Western Blot analysis of cell lysates (20µg) using ab16753 at 1/1000.

    Lane 1: HeLa cell lysates
    Lane 2: 293T cell lysates
    Lane 3: Jurkat cell lysates

    Secondary antibody conjugated to HRP

  • IHC image of ab16753 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16753, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Peroxiredoxin 3 antibody [4G10] (ab16753)参考文献

This product has been referenced in:
  • Maxwell GL  et al. Proteomic analysis of stage I endometrial cancer tissue: Identification of proteins associated with oxidative processes and inflammation. Gynecol Oncol : (2011). WB ; Human . Read more (PubMed: 21458040) »
  • Kim SY  et al. H2O2-dependent hyperoxidation of peroxiredoxin 6 (Prdx6) plays a role in cellular toxicity via up-regulation of iPLA2 activity. J Biol Chem 283:33563-8 (2008). ICC/IF ; Human . Read more (PubMed: 18826942) »

See all 3 Publications for this product

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