Anti-Peroxiredoxin 2抗体(ab15572)


  • 产品名称Anti-Peroxiredoxin 2抗体
    参阅全部 Peroxiredoxin 2 一抗
  • 描述
    兔多克隆抗体to Peroxiredoxin 2
  • 经测试应用适用于: IHC-P, ICC/IF, WB, ICCmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Peroxiredoxin 2 aa 104-115.


  • 阳性对照
    • TSU PR1 cells (ICC) PC3 cell lysate (WB)



Our Abpromise guarantee covers the use of ab15572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).
ICC 1/500.


  • 功能Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • 序列相似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Epididymis secretory sperm binding protein Li 2a antibody
    • HEL S 2a antibody
    • MGC4104 antibody
    • Natural killer cell enhancing factor B antibody
    • Natural killer cell-enhancing factor B antibody
    • Natural Killer Enhancing Factor B antibody
    • NKEF B antibody
    • NKEF-B antibody
    • NKEFB antibody
    • Peroxiredoxin-2 antibody
    • PRDX 2 antibody
    • PRDX2 antibody
    • PRDX2_HUMAN antibody
    • PrP antibody
    • PRX2 antibody
    • PRXII antibody
    • PTX1 antibody
    • TDPX1 antibody
    • Thiol Specific Antioxidant 1 antibody
    • Thiol specific antioxidant protein antibody
    • Thiol-specific antioxidant protein antibody
    • Thioredoxin Dependent Peroxide Reductase 1 antibody
    • Thioredoxin peroxidase 1 antibody
    • Thioredoxin-dependent peroxide reductase 1 antibody
    • Torin antibody
    • TPX1 antibody
    • TSA antibody
    see all

Anti-Peroxiredoxin 2 antibody 图像

  • Staining of Peroxiredoxin 2 in TSU PR1 cells using ab15572.
  • ICC/IF image of ab15572 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15572, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab15572 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15572, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Peroxiredoxin 2 antibody (ab15572)参考文献

This product has been referenced in:
  • Yang J  et al. Site-specific mapping and quantification of protein S-sulphenylation in cells. Nat Commun 5:4776 (2014). Read more (PubMed: 25175731) »
  • Lichtenfels R  et al. Comparative expression profiling of distinct T cell subsets undergoing oxidative stress. PLoS One 7:e41345 (2012). WB ; Human . Read more (PubMed: 22911781) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 20000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (Jurkats)
Specification Jurkats
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. Tejpal Gill

Verified customer

提交于 Jan 10 2014

Based on information from the originator, this antibody is specific for peroxiredoxin 2 and does not show cross-reactivity with other isoforms.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEPG2, HEK293)
Loading amount 10 µg
Specification HEPG2, HEK293
Blocking step BSA and non fat dry milk as blocking agent for 30 minute(s) · Concentration: 3%, 2%

Abcam user community

Verified customer

提交于 Nov 30 2006

Thank you for your enquiry. I have performed an alignment of the immunogen seqeunces used to raise ab15571 and ab15572 with the sequence you have provided me with. It is available at the link below for the next 24 hours.

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This antibody does not contain total Ig or buffer because it is antiserum. Since it is antiserum it is also not purified. This antibody cannot be supplied without the sodium azide, my apologies for the inconvenience. Please do not hesitate to...

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