使用敲除细胞株进行验证RabMAb

Anti-Peroxiredoxin 1抗体[EPR5433] (ab109498)

概述

  • 产品名称Anti-Peroxiredoxin 1抗体[EPR5433]
    参阅全部 Peroxiredoxin 1 一抗
  • 描述
    兔单克隆抗体[EPR5433] to Peroxiredoxin 1
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Peroxiredoxin 1

  • 阳性对照
    • WB: MCF-7, 293T, and K562 cell lysates IHC-P: Kidney tissue , Thyroid carcinoma tissue IF: 293T cells
  • 常规说明

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/250 - 1/500. Perform antigen retrieval
Flow Cyt 1/10 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
    • 序列相似性Belongs to the ahpC/TSA family.
      Contains 1 thioredoxin domain.
    • 翻译后修饰Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
    • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Heme binding 23 kDa protein antibody
      • MSP23 antibody
      • Natural killer cell-enhancing factor A antibody
      • NKEF A antibody
      • NKEF-A antibody
      • NKEFA antibody
      • OSF3 antibody
      • Osteoblast specific factor 3 antibody
      • PAG antibody
      • Paga antibody
      • PAGB antibody
      • Peroxiredoxin-1 antibody
      • PRDX1 antibody
      • PRDX1_HUMAN antibody
      • Proliferation associated gene A antibody
      • Proliferation-associated gene protein antibody
      • PRX1 antibody
      • PrxI antibody
      • TDPX2 antibody
      • Thioredoxin peroxidase 2 antibody
      • Thioredoxin-dependent peroxide reductase 2 antibody
      see all

    Anti-Peroxiredoxin 1 antibody [EPR5433] 图像



    • Predicted band size : 22 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Peroxiredoxin knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109498 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109498 was shown to specifically react with Peroxiredoxin when Peroxiredoxin knockout samples were used. Wild-type and Peroxiredoxin knockout samples were subjected to SDS-PAGE. ab109498 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Overlay histogram showing HeLa cells stained with ab109498 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109498, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Peroxiredoxin 1 antibody [EPR5433] (ab109498) at 1/10000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 22 kDa
      Observed band size : 22 kDa
    • Immunohistochemical analysis of paraffin-embedded kidney tissue using ab109498 at 1/250
    • Immunohistochemical analysis of paraffin-embedded thyroid carcinoma tissue using ab109498 at 1/250.
    • Immunofluorescent analysis of 293T cells labeled with ab109498 at 1/10.

    Anti-Peroxiredoxin 1 antibody [EPR5433] (ab109498)参考文献

    This product has been referenced in:
    • Riquier S  et al. Peroxiredoxin post-translational modifications by redox messengers. Redox Biol 2:777-85 (2014). WB ; Mouse . Read more (PubMed: 25009779) »
    • Gambari L  et al. Sodium hydrosulfide inhibits the differentiation of osteoclast progenitor cells via NRF2-dependent mechanism. Pharmacol Res 87:99-112 (2014). Human . Read more (PubMed: 24998607) »

    See all 2 Publications for this product

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