使用敲除细胞株进行验证

Anti-Peroxiredoxin 1抗体[3G5] (ab70666)

概述

  • 产品名称Anti-Peroxiredoxin 1抗体[3G5]
    参阅全部 Peroxiredoxin 1 一抗
  • 描述
    小鼠单克隆抗体[3G5] to Peroxiredoxin 1
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Recombinant full length Human Peroxiredoxin 1 purified from E.coli

  • 阳性对照
    • HeLa, A431, HL-60 and SK-N-MC cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab70666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IP Use at 7.5 µg/mg of lysate.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • 序列相似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 翻译后修饰Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heme binding 23 kDa protein antibody
    • MSP23 antibody
    • Natural killer cell-enhancing factor A antibody
    • NKEF A antibody
    • NKEF-A antibody
    • NKEFA antibody
    • OSF3 antibody
    • Osteoblast specific factor 3 antibody
    • PAG antibody
    • Paga antibody
    • PAGB antibody
    • Peroxiredoxin-1 antibody
    • PRDX1 antibody
    • PRDX1_HUMAN antibody
    • Proliferation associated gene A antibody
    • Proliferation-associated gene protein antibody
    • PRX1 antibody
    • PrxI antibody
    • TDPX2 antibody
    • Thioredoxin peroxidase 2 antibody
    • Thioredoxin-dependent peroxide reductase 2 antibody
    see all

Anti-Peroxiredoxin 1 antibody [3G5] 图像



  • Predicted band size : 22 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Peroxiredoxin knockout HAP1 cell lysate (20 µg)
    Lane 3: A431 cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab70666 observed at 23 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab70666 was shown to specifically react with Peroxiredoxin when Peroxiredoxin knockout samples were used. Wild-type and Peroxiredoxin knockout samples were subjected to SDS-PAGE. ab70666 and ab181602 (loading control to GAPDH) were diluted 1/20 000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-Peroxiredoxin 1 antibody [3G5] (ab70666) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HL-60 cell lysate
    Lane 4 : SK-N-MC cell lysate


    Predicted band size : 22 kDa
    Observed band size : 22 kDa

Anti-Peroxiredoxin 1 antibody [3G5] (ab70666)参考文献

ab70666 has not yet been referenced specifically in any publications.

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