Anti-Peroxidase anti-Peroxidase complex / PAP抗体(ab21867)

概述

  • 产品名称Anti-Peroxidase anti-Peroxidase complex / PAP抗体
  • 描述
    小鼠多克隆抗体to Peroxidase anti-Peroxidase complex / PAP
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Fusion protein:

    STSGGPPAKQLGMTSAISLAEPRPEDLQRTDELRGSLEPYNVFESQDELN HRMEILAKLNTLVKQWVKEISVSKNMPESAAEKLGGKIYTFGSYRLGVHH

    , corresponding to amino acids 18/117 of Drosophila melanogaster PAP.

  • 常规说明

    Produced from outbred CD1 mice

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Constituents: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 74 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性Peroxidase anti-Peroxidase (PAP) complexes are formed by antibodies raised towards Horse Radish Peroxidase (HRP), binding to HRP molecules. Such complexes are used as tertiary detection / signal amplification reagents in immunochemical staining techniques. It must be emphasised that the primary antibody must be raised in the same species as the antibodies used to form the PAP complex, so that a secondary "linker" antibody can be used to "bridge" the primary antibody to the PAP complex. PAP complexes provide a sensitive means of signal amplification, more so than standard direct conjugation of the label to a primary, secondary or tertiary antibody, but not to the degree of avidin / biotin based methods. PAP complexes owe their increased sensitivity to the fact that binding to HRP is facilitated by the antibody's natural affinity for HRP, in oppose to chemical conjugation.
  • 数据库链接
    • 别名
      • Hiiragi CG9854-PA antibody
      • hrg antibody
      • PAP antibody
      • peroxidase antiperoxidase antibody
      • Poly A polymerase antibody
      see all

    Anti-Peroxidase anti-Peroxidase complex / PAP antibody 图像

    • All lanes : Anti-Peroxidase anti-Peroxidase complex / PAP antibody (ab21867) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 74 kDa

    Anti-Peroxidase anti-Peroxidase complex / PAP antibody (ab21867)参考文献

    ab21867 has not yet been referenced specifically in any publications.

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