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Synthetic non-phosphopeptide derived from human PERK around the phosphorylation site of threonine 981 (RHTPGQ)
Our Abpromise guarantee covers the use of ab79483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 125 kDa.|
|IHC-P||1/50 - 1/100.|
IHC-P image of PERK staining on human cortical sections using ab79483 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab79483 was diluted 1:500 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)
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