Anti-PERK抗体(ab79483)
Key features and details
- Rabbit polyclonal to PERK
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PERK抗体
参阅全部 PERK 一抗 -
描述
兔多克隆抗体to PERK -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic non-phosphopeptide derived from human PERK around the phosphorylation site of threonine 981 (RHTPGQ)
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阳性对照
- IHC: human colon carcinoma tissue WB: extracts from MCF-7 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (4) |
1/500 - 1/1000. Predicted molecular weight: 125 kDa.
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IHC-P | (1) |
1/50 - 1/100.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 125 kDa. |
IHC-P
1/50 - 1/100. |
靶标
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功能
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. -
组织特异性
Ubiquitous. A high level expression is seen in secretory tissues. -
疾病相关
Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain. -
结构域
The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. -
翻译后修饰
Autophosphorylated.
N-glycosylated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9451 Human
- Entrez Gene: 13666 Mouse
- Entrez Gene: 29702 Rat
- Omim: 604032 Human
- SwissProt: Q9NZJ5 Human
- SwissProt: Q9Z2B5 Mouse
- SwissProt: Q9Z1Z1 Rat
- Unigene: 591589 Human
see all -
别名
- DKFZp781H1925 antibody
- E2AK3_HUMAN antibody
- EC 2.7.11.1 antibody
see all
图片
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All lanes : Anti-PERK antibody (ab79483) at 1/500 dilution
Lane 1 : extracts from MCF-7 cells
Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 125 kDa
Observed band size: 125 kDa -
IHC-P image of PERK staining on human cortical sections using ab79483 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab79483 was diluted 1:500 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, stained with ab79483 at 1/50-1/100 dilution, in the absence and presence of the immunising peptide
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab79483 被引用在 22 文献中.
- Zhang Y et al. Azithromycin alleviates the severity of rheumatoid arthritis by targeting the unfolded protein response component of glucose-regulated protein 78 (GRP78). Br J Pharmacol 179:1201-1219 (2022). PubMed: 34664264
- Li S et al. Sarco/endoplasmic reticulum Ca2+ -ATPase (SERCA2b) mediates oxidation-induced endoplasmic reticulum stress to regulate neuropathic pain. Br J Pharmacol 179:2016-2036 (2022). PubMed: 34811737
- Wang Y et al. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells. Open Med (Wars) 17:1780-1787 (2022). PubMed: 36447525
- Otero A et al. Prion-Associated Neurodegeneration Causes Both Endoplasmic Reticulum Stress and Proteasome Impairment in a Murine Model of Spontaneous Disease. Int J Mol Sci 22:N/A (2021). PubMed: 33466523
- Li X et al. Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2a/ATF4/CHOP pathway in breast cancer. Cell Death Dis 12:314 (2021). PubMed: 33762577