Anti-PERK抗体(ab79483)

概述

  • 产品名称Anti-PERK抗体
    参阅全部 PERK 一抗
  • 描述
    兔多克隆抗体to PERK
  • 特异性ab79483 detects endogenous levels of total PERK protein
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human PERK around the phosphorylation site of threonine 981 (RHTPGQ)

  • 阳性对照
    • IHC: human colon carcinoma tissue WB: extracts from MCF-7 cells

性能

应用

Our Abpromise guarantee covers the use of ab79483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 125 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
  • 组织特异性Ubiquitous. A high level expression is seen in secretory tissues.
  • 疾病相关Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 1 protein kinase domain.
  • 结构域The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.
  • 翻译后修饰Autophosphorylated.
    N-glycosylated.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781H1925 antibody
    • E2AK3_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak3 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 3 antibody
    • Heme regulated EIF2 alpha kinase antibody
    • HRI antibody
    • HsPEK antibody
    • Pancreatic eIF2 alpha kinase antibody
    • Pancreatic eIF2-alpha kinase antibody
    • PEK antibody
    • PRKR like endoplasmic reticulum kinase antibody
    • PRKR-like endoplasmic reticulum kinase antibody
    • WRS antibody
    see all

Anti-PERK antibody 图像

  • IHC-P image of PERK staining on human cortical sections using ab79483 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab79483 was diluted 1:500 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)

    See Abreview

  • All lanes : Anti-PERK antibody (ab79483) at 1/500 dilution

    Lane 1 : extracts from MCF-7 cells
    Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 125 kDa
    Observed band size : 125 kDa
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, stained with ab79483 at 1/50-1/100 dilution, in the absence and presence of the immunising peptide

Anti-PERK antibody (ab79483)参考文献

ab79483 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Cortical section)
Specification Cortical section
Permeabilization Yes - Triton-X
Fixative Formaldehyde
Username

Dr. Martin Broadstock

Verified customer

提交于 Feb 21 2014

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Heart, skeletal muscle, liver)
Loading amount 60 µg
Specification Heart, skeletal muscle, liver
Gel Running Conditions Non-reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

提交于 Dec 21 2012

Thank you for your phone call. Please find you discount code and the conditions of use below:

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab79483 in mouse. This...

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Thank you for providing that information.

As I mentioned previously, with the exception of ab79483, we do guarantee that these antibodies should work in western blot on rat tissue. If we cannot resolve the issue you are having with the antibod...

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Thank you for contacting Abcam.

I am sorry that you are having some issues with our antibodies. All of the antibodies you mentioned should work in western blot in various species, however only ab30315 is known to work in IHC-P. Below is a list...

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Application Western blot
Sample Rat Cell lysate - whole cell (glioma)
Loading amount 20 µg
Specification glioma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 04 2012

Thank you for your call today and for your questions. As we discussed, I would be happy to help resolve the issues with the antibodies that you have purchased. Could you please send the following information about the protocol and results using ab79483...

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DISCOUNT CODES: ab96481 : ******** ab79483 : ******** ab25898 : ******** Expiration date: DD MM YYYY I am very pleased to hear you would like to accept our offer and test these antibodies in rat. This code will give you 1 free primary for eac...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"