Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
All lanes : Anti-Peripherin antibody (ab99942) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 54 kDa Observed band size : 54 kDa Additional bands at : 14 kDa,40 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Peripherin antibody (ab99942)This image is courtesy of an Abreview submitted by Karine Thibault
ab99942 staining rat spinal cord sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected with 30% sucrose and sectioned using a cryostat. Staining with ab99942 at a 1/1000 dilution in PBS-Triton (0.3%) with 0.02% azide was performed for 18h at 25°C. A donkey anti-rabbit Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody.
ICC/IF image of ab99942 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99942 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.