Anti-Peripherin抗体(ab99942)

概述

  • 产品名称
    Anti-Peripherin抗体
    参阅全部 Peripherin 一抗
  • 描述
    兔多克隆抗体to Peripherin
  • 经测试应用
    适用于: ICC/IF, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Cow, Human, Pig
  • 免疫原

    Synthetic peptide within Mouse Peripherin aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab111786)

  • 阳性对照
    • WB: Mouse brain and spinal cord tissue lysates. ICC/IF: PC12 cells.

性能

应用

Our Abpromise guarantee covers the use of ab99942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

靶标

Anti-Peripherin antibody 图像

  • All lanes : Anti-Peripherin antibody (ab99942) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 14 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab99942 staining rat spinal cord sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected with 30% sucrose and sectioned using a cryostat. Staining with ab99942 at a 1/1000 dilution in PBS-Triton (0.3%) with 0.02% azide was performed for 18h at 25°C. A donkey anti-rabbit Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab99942 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99942 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Peripherin antibody (ab99942)参考文献

ab99942 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Dog Cultured Cells (Skin derived neurons)
Permeabilization
Yes - 0.1% Triton-X
Specification
Skin derived neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 12 2017

Application
Immunocytochemistry
Sample
Mouse Cell (DRG neuron)
Specification
DRG neuron
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 May 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Karine Thibault

Verified customer

提交于 Oct 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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