概述

  • 产品名称Anti-Peripherin抗体
    参阅全部 Peripherin 一抗
  • 描述
    鸡多克隆抗体to Peripherin
  • 经测试应用适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant protein expressed in bacteria.

性能

应用

Our Abpromise guarantee covers the use of ab39374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.
IHC-Fr 1/50 - 1/100.

靶标

Anti-Peripherin antibody 图像

  • ab39374 staining Peripherin in Human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/250 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor® 488-conjugated Goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.

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  • Ab39374 staining human peripherin in neonatal rat cortical neurons cultured for 14-21 days by ICC/IF.
  • ab39374 at a 1/100 dilution staining Peripherin in rat brain tissue (cerebellum) by Immunohistochemistry (PFA fixed, frozen sections), incubated for 12 hours at 4°C. Permeabilized using 0.3% Triton. Blocked with 10% donkey serum for 1 hour at 22°C. Secondary used at 1/500 polyclonal Goat Vector BA 9010 conjugated to biotin. Biotin detected with streptavidin Alexa Fluor 594.
    The image shows a climbing fiber in the cerebellar molecular layer.

    See Abreview

Anti-Peripherin antibody (ab39374)参考文献

This product has been referenced in:

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6.0
Sample Human Tissue sections (Spiral ganglion)
Specification Spiral ganglion
Permeabilization Yes - Tween-20
Fixative Paraformaldehyde
Username

Mr. Heiko Locher

Verified customer

提交于 Sep 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain, cerebellum)
Specification Brain, cerebellum
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton
Blocking step Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"