Anti-Peripherin抗体[8G2] (ab4653)


  • 产品名称Anti-Peripherin抗体[8G2]
    参阅全部 Peripherin 一抗
  • 描述
    小鼠单克隆抗体[8G2] to Peripherin
  • 经测试应用适用于: WB, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Recombinant Rat Peripherin purified from E. coli.



Our Abpromise guarantee covers the use of ab4653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-Fr Use at an assay dependent concentration. ab4653 is relatively sensitive to aldehyde fixation and so is not recommended for heavily fixed formalin fixed sections of brain.
ICC/IF 1/500.


Anti-Peripherin antibody [8G2] 图像

  • Predicted band size : 57 kDa
    Cytoskeletal homogenates of rat spinal cord were run out on a 7.5% SDS-PAGE gel and stained with Coomassie brilliant blue (lane 1). H, M, L and G indicate the positions of neurofilament NF-H, NF-M, NF-L and glial fibrillary acidic protein respectively. These proteins, as expressed in rat, have SDS-PAGE molecular weights of 200kDa, 145kDa, 68kDa and 50kDa respectively. The same material was run out on a single wide lane on an SDS-PAGE gel and transferred to nitrocellulose. Individual strips cut from this membrane were probed with monoclonal antibody to peripherin (lane 2, ab4653), or polyclonal antibody to peripherin (lane 3, ab4666). Vimentin, a protein related to peripherin in protein sequence and structure, is also found in these preparations since it is found in astrocytes and endothelial cells. Vimentin runs a little faster on SDS-PAGE gels, as revealed with an antibody to this protein (Lane 4).
  • Immunohistochemical analysis of rat dorsal root ganglia, staining Peripherin with ab4653.

    The DRGs from sham rats were cut to 30 μm and collected free floating in 0.1 m PBS. Sections were blocked with 5% blocking reagent for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) and an Alexa Fluor®594-conjugated donkey anti-mouse IgG (1/400) was used as the secondary antibody.

Anti-Peripherin antibody [8G2] (ab4653)参考文献

This product has been referenced in:
  • Li L  et al. Up-regulation of Cavß3 subunit in primary sensory neurons increases voltage-activated Ca2+ channel activity and nociceptive input in neuropathic pain. J Biol Chem 287:6002-13 (2012). IHC-FrFl ; Rat . Read more (PubMed: 22187436) »
  • Brandao KE  et al. A-kinase anchoring protein 150 expression in a specific subset of TRPV1- and CaV 1.2-positive nociceptive rat dorsal root ganglion neurons. J Comp Neurol 520:81-99 (2012). WB, ICC/IF ; Rat . Read more (PubMed: 21674494) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization No

Dr. Karine Thibault

Verified customer

提交于 Oct 17 2011

Thank you for your enquiry. Both antibodies do stain cerebellar climbing fibers; please see: Errante et al. The intermediate filament protein peripherin is a marker for cerebellar climbing fibres. J Neurocytol. 1998 Feb;27(2):69-84. PubMed ID 960...

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