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Our Abpromise guarantee covers the use of ab4653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|IHC-Fr||Use at an assay dependent concentration. ab4653 is relatively sensitive to aldehyde fixation and so is not recommended for heavily fixed formalin fixed sections of brain.|
Immunofluorescent analysis of human neuroblastoma cell line SHSY5Y (A) and rat pheochromocytoma cell line PC12 (B), stained with ab4653 at dilution 1:500, in red, and costained with chicken pAb to vimentin, at dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA.
Immunohistochemical analysis of rat dorsal root ganglia, staining Peripherin with ab4653.
The DRGs from sham rats were cut to 30 μm and collected free floating in 0.1 m PBS. Sections were blocked with 5% blocking reagent for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) and an Alexa Fluor®594-conjugated donkey anti-mouse IgG (1/400) was used as the secondary antibody.
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