Anti-Periostin抗体(ab79946)
Key features and details
- Rabbit polyclonal to Periostin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Periostin抗体
参阅全部 Periostin 一抗 -
描述
兔多克隆抗体to Periostin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chicken, Cow -
免疫原
Synthetic peptide corresponding to Human Periostin aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90847) -
阳性对照
- This antibody gave a positive signal in WB in Human breast tumour tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast fibroadenocarcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79946于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (2) |
Use a concentration of 0.1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 80, 86, 86, 93 kDa (predicted molecular weight: 93 kDa). This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 & 4 of Human Periostin.
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-P
Use a concentration of 0.1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80, 86, 86, 93 kDa (predicted molecular weight: 93 kDa). This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 & 4 of Human Periostin. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
组织特异性
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
序列相似性
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
翻译后修饰
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 10631 Human
- Entrez Gene: 50706 Mouse
- Omim: 608777 Human
- SwissProt: Q15063 Human
- SwissProt: Q62009 Mouse
- Unigene: 136348 Human
- Unigene: 721018 Human
- Unigene: 236067 Mouse
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别名
- Fasciclin-I like antibody
- MGC119510 antibody
- MGC119511 antibody
see all
图片
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ab79946 staining Periostin in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab79946 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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IHC image of Periostin staining in Human breast fibroadenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79946, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Anti-Periostin antibody (ab79946) at 1 µg/ml (blocked with 3% milk) + Breast (Human) Cytoplasmic Lysate - tumor tissue (ab30085) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 80,80,86,87,93 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 & 4 of Human Periostin. The predicted molecular weights of isoforms 1,2,3 & 4 are 93, kDa, 87 kDa, 87 kDa and 83 kDa respectively.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab79946 被引用在 10 文献中.
- Li X et al. Single-cell RNA sequencing reveals a pro-invasive cancer-associated fibroblast subgroup associated with poor clinical outcomes in patients with gastric cancer. Theranostics 12:620-638 (2022). PubMed: 34976204
- Müller C et al. Protein Signatures of Remodeled Airways in Transplanted Lungs with Bronchiolitis Obliterans Syndrome Obtained Using Laser-Capture Microdissection. Am J Pathol 191:1398-1411 (2021). PubMed: 34111430
- Elowsson Rendin L et al. Matrisome Properties of Scaffolds Direct Fibroblasts in Idiopathic Pulmonary Fibrosis. Int J Mol Sci 20:N/A (2019). PubMed: 31426504
- Meng X et al. Periostin has a protective role in melatonin-induced cell apoptosis by inhibiting the eIF2a-ATF4 pathway in human osteoblasts. Int J Mol Med 41:1003-1012 (2018). PubMed: 29207036
- Zhang Q et al. Cancer-associated stroma significantly contributes to the mesenchymal subtype signature of serous ovarian cancer. Gynecol Oncol N/A:N/A (2018). PubMed: 30448260
- Wei Y et al. Excessive periostin expression and Th2 response in patients with nasal polyps: association with asthma. J Thorac Dis 10:6585-6597 (2018). PubMed: 30746204
- Sverrild A et al. Eosinophilic airway inflammation in asthmatic patients is associated with an altered airway microbiome. J Allergy Clin Immunol 140:407-417.e11 (2017). PubMed: 28042058
- Nagaraju CK et al. Global fibroblast activation throughout the left ventricle but localized fibrosis after myocardial infarction. Sci Rep 7:10801 (2017). PubMed: 28883544
- Guo X et al. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. Oncotarget 7:80521-80542 (2016). WB ; Human . PubMed: 27602954
- Kong P et al. Lack of specificity of fibroblast-specific protein 1 in cardiac remodeling and fibrosis. Am J Physiol Heart Circ Physiol 305:H1363-72 (2013). IHC ; Mouse . PubMed: 23997102