Anti-Periostin抗体(ab14041)
Key features and details
- Rabbit polyclonal to Periostin
- Suitable for: ELISA, ICC/IF, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Periostin抗体
参阅全部 Periostin 一抗 -
描述
兔多克隆抗体to Periostin -
宿主
Rabbit -
经测试应用
适用于: ELISA, ICC/IF, IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Human -
免疫原
Recombinant fragment (His-tag) corresponding to Human Periostin (N terminal).
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阳性对照
- IHC-P: Human colon and normal skin tissues; Prostate cancer tissues. ICC/IF: HeLa cells.
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常规说明
Lots # GR310924-1, GR296166-4, and GR296166-5 are sold in lyophilized form. To reconstitute, add 100 µL of deionized water and let the lyophilized antibody dissolve completely. The resulting concentration is 0.5 mg/mL. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: PBS, 0.58% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14041于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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ICC/IF | (3) |
Use at an assay dependent concentration.
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IHC-Fr | (6) |
Use at an assay dependent concentration. PubMed: 19079190
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IHC-P | (8) |
1/100 - 1/1000.
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说明 |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 19079190 |
IHC-P
1/100 - 1/1000. |
靶标
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功能
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
组织特异性
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
序列相似性
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
翻译后修饰
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 10631 Human
- Entrez Gene: 50706 Mouse
- Entrez Gene: 361945 Rat
- Omim: 608777 Human
- SwissProt: Q15063 Human
- SwissProt: Q62009 Mouse
- Unigene: 136348 Human
- Unigene: 721018 Human
see all -
别名
- Fasciclin-I like antibody
- MGC119510 antibody
- MGC119511 antibody
see all
图片
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Immunohistochemistry analysis of formalin-fixed paraffin-embedded human colon tissue section labeling Periostin with ab14041.
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Immunohistochemistry analysis of formalin-fixed paraffin-embedded human normal skin tissue sectionlabeling Periostin with ab14041.
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The periostin staining in prostate cancer and paired non-tumorous prostate tissues (TMA from JHU, 140 cases).
(A) The pathologists’ criteria for IHC scoring.(a) 0, undetectable; (b)1+, weak staining; (c) 2+, medium staining; (d) 3+, strong staining. Periostin was mainly stained in peritumoral stroma.
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ICC/IF image of ab14041 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (193)
ab14041 被引用在 193 文献中.
- Daghrery A et al. Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues. Bioact Mater 19:268-281 (2023). PubMed: 35574052
- Fleming Martinez AK et al. Ym1+ macrophages orchestrate fibrosis, lesion growth, and progression during development of murine pancreatic cancer. iScience 25:104327 (2022). PubMed: 35602933
- Jang SY et al. Therapeutic Potential of Targeting Periostin in the Treatment of Graves' Orbitopathy. Front Endocrinol (Lausanne) 13:900791 (2022). PubMed: 35707463
- Miyai K et al. Prognostic impact of stromal periostin expression in upper urinary tract urothelial carcinoma. BMC Cancer 22:787 (2022). PubMed: 35850759
- Tsukasaki M et al. Periosteal stem cells control growth plate stem cells during postnatal skeletal growth. Nat Commun 13:4166 (2022). PubMed: 35851381