Anti-Perilipin A+B抗体(ab66514)

概述

  • 产品名称Anti-Perilipin A+B抗体
    参阅全部 Perilipin A+B 一抗
  • 描述
    兔多克隆抗体to Perilipin A+B
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: MEPVVRRLST QFTAANELAC RGLDHLEEKI PALQYPPEKI ASELKDTIST, corresponding to a region within N terminal amino acids 73-122 of Human Perilipin A+B

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab66514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay:1:312500.

靶标

  • 相关性Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
  • 细胞定位Lipid droplet
  • 数据库链接
  • 别名
    • Lipid droplet associated protein antibody
    • PERIA antibody
    • PERIB antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin B antibody
    • PLIN antibody
    see all

Anti-Perilipin A+B antibody 图像

  • Anti-Perilipin A+B antibody (ab66514) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

Anti-Perilipin A+B antibody (ab66514)参考文献

ab66514 has not yet been referenced specifically in any publications.

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