Anti-Perilipin-1抗体(ab87129)

概述

  • 产品名称
    Anti-Perilipin-1抗体
    参阅全部 Perilipin-1 一抗
  • 描述
    兔多克隆抗体to Perilipin-1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide within Rat Perilipin-1 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab109636)

  • 阳性对照
    • WB: Mouse brown adipose tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab87129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 56 kDa).

靶标

  • 功能
    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • 组织特异性
    Adipocytes.
  • 序列相似性
    Belongs to the perilipin family.
  • 翻译后修饰
    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • 细胞定位
    Lipid droplet. Lipid droplet surface-associated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Lipid droplet associated protein antibody
    • Lipid droplet-associated protein antibody
    • PERI antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin-1 antibody
    • PerilipinA antibody
    • PLIN antibody
    • PLIN1 antibody
    • PLIN1_HUMAN antibody
    see all

Anti-Perilipin-1 antibody 图像

  • Anti-Perilipin-1 antibody (ab87129) at 1 µg/ml + Adult Mouse Brown Adipose Tissue Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    The predicted molecular weight of Perilipin-1 is 56 kDa (SwissProt), however we expect to observe a banding pattern at 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

实验方案

Anti-Perilipin-1 antibody (ab87129)参考文献

ab87129 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - other (Duodenum Cytosolic Lipid Dropets)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
30 µg
Specification
Duodenum Cytosolic Lipid Dropets
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 May 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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