概述

  • 产品名称
    Anti-Perilipin-1抗体
    参阅全部 Perilipin-1 一抗
  • 描述
    山羊多克隆抗体to Perilipin-1
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human Perilipin-1 (C terminal).
    Sequence:

    EPILGRAQYSQLRKK

  • 阳性对照
    • WB: Human adipose lysate. ICC/IF: Differentiated 3T3-L1 adipocytes.

性能

应用

Our Abpromise guarantee covers the use of ab61682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能
    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • 组织特异性
    Adipocytes.
  • 序列相似性
    Belongs to the perilipin family.
  • 翻译后修饰
    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • 细胞定位
    Lipid droplet. Lipid droplet surface-associated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Lipid droplet associated protein antibody
    • Lipid droplet-associated protein antibody
    • PERI antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin-1 antibody
    • PerilipinA antibody
    • PLIN antibody
    • PLIN1 antibody
    • PLIN1_HUMAN antibody
    see all

Anti-Perilipin-1 antibody 图像

  • ab61682 staining Perilipin-1 in human adipose tissue sections by Immunohistochemistry (IHC-P - Formalin/PFA-fixed, paraffin-embedded sections). Tissue was fixed with 10% buffred formalin and blocked with 5% serum for 1 hour at 4°C; antigen retrieval was by heat mediation in 10mM sodium citrate buffer, pH 6. Samples were incubated with the primary antibody (1/100) for 12 hours at 4°C. A Cy3®-conjugated donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Anti-Perilipin-1 antibody (ab61682) at 0.01 µg/ml + Human Adipose lysate at 35 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab61682 staining Perilipin-1 in mouse adipose tissue sections by Immunohistochemistry (IHC-P - Formalin/PFA-fixed, paraffin-embedded sections). Tissue was fixed with 10% buffred formalin and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in 10mM sodium citrate buffer, pH 6. Samples were incubated with the primary antibody (1/100) for 12 hours at 4°C. A Cy3®-conjugated donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Small lipid droplets preferentially stained in the periphery of differentiated 3T3-L1 adipocytesby ab61682 at a dilution of 1ug/ml.

Anti-Perilipin-1 antibody (ab61682)参考文献

This product has been referenced in:
  • Pearring JN  et al. Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration. PLoS Genet 13:e1006740 (2017). Read more (PubMed: 28410364) »
  • Matthews AG  et al. Compound heterozygous mutation of Rag1 leading to Omenn syndrome. PLoS One 10:e0121489 (2015). Read more (PubMed: 25849362) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brown adipocyte)
Permeabilization
Yes - 0.05% TX-100
Specification
Brown adipocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
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Verified customer

提交于 Sep 26 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
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提交于 Sep 01 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Adipose tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate buffer pH6
Permeabilization
No
Specification
Adipose tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
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提交于 Nov 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Adipose tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Adipose tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
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提交于 Nov 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Adipose tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6
Permeabilization
No
Specification
Adipose tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
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提交于 Nov 09 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adipose tissue)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Adipose tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
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提交于 Nov 05 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Hoffa's fat pat)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: buffer citrate
Permeabilization
No
Specification
Hoffa's fat pat
Blocking step
5% horse serum 4%BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
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提交于 Feb 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adipose tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citric PH6.0
Permeabilization
No
Specification
adipose tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Fixative
Formaldehyde
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Verified customer

提交于 Feb 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human cells in Matrigel)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris-based pH9
Permeabilization
No
Specification
human cells in Matrigel
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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