Anti-Pericentrin抗体- Centrosome Marker (ab4448)

概述

  • 产品名称
    Anti-Pericentrin抗体- Centrosome Marker
    参阅全部 Pericentrin 一抗
  • 描述
    兔多克隆抗体to Pericentrin - Centrosome Marker
  • 特异性
    This antibody should recognise both Pericentrin and Kendrin (also known as Pericentrin-2).
  • 经测试应用
    适用于: ICC/IF, IHC-P, ICC, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human, African green monkey
  • 免疫原

    The pericentrin clone used is 1.7 kb in size and is derived from within residues 100-600 of mouse pericentrin 1. It was expressed as a fusion protein. The corresponding amino acids are present in both pericentrin and kendrin (pericentrin-2) so this antibody is predicted to cross-react with both isoforms.

  • 阳性对照
    • ICC/IF: MCF7 and NIH3T3 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 0.1 - 0.5 µg/ml.
IHC-P 1/4500.
ICC Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能
    Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
  • 组织特异性
    Expressed in all tissues tested, including placenta, liver, kidney and thymus.
  • 疾病相关
    Microcephalic osteodysplastic primordial dwarfism 2
  • 结构域
    Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
  • 细胞定位
    Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Centrosome Marker antibody
    • Ken antibody
    • Kendrin antibody
    • KIAA0402 antibody
    • MOPD2 antibody
    • PCN antibody
    • PCNT 2 antibody
    • PCNT antibody
    • PCNT B antibody
    • PCNT_HUMAN antibody
    • PCNT1 antibody
    • PCNT2 antibody
    • PCNTB antibody
    • PCTN2 antibody
    • Pericentrin 1 antibody
    • Pericentrin 2 antibody
    • Pericentrin 380 antibody
    • Pericentrin antibody
    • Pericentrin B antibody
    • Pericentrin-B antibody
    • SCKL4 antibody
    see all

Anti-Pericentrin antibody - Centrosome Marker 图像

  • ICC/IF image of ab4448 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4448, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • ab4448 stained NIH-3T3 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • NIH3T3 cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature. The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.

  • Image courtesy of Human Protein Atlas

        Paraffin embedded human kidney tissue sections were incubated with ab4448 (1/4500 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab4448 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org

  • IF staining of pericentrin in MCF7 cells (human).

    The top panel is an interphase cell showing centrosome staining.  The bottom panel shows a mitotic cell with spindle pole staining.  ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).

    Top panel - 630X magnification; Bottom panel -1000X magnification. The secondary antibody was Alexa488 anti-rabbit.

  • ICC/IF image of ab4448 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Pericentrin antibody - Centrosome Marker (ab4448)参考文献

This product has been referenced in:
  • Subramanian L  et al. Dynamic behaviour of human neuroepithelial cells in the developing forebrain. Nat Commun 8:14167 (2017). IHC-Fr ; Human . Read more (PubMed: 28139695) »
  • Rickardson L  et al. Evaluation of the antitumor activity of NOV202, a novel microtubule targeting and vascular disrupting agent. Drug Des Devel Ther 11:1335-1351 (2017). ICC/IF ; Chicken . Read more (PubMed: 28496304) »

See all 149 Publications for this product

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Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (4-12 Bis tris)
Loading amount
5e+006 cells
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Mar 18 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT1080)
Permeabilization
Yes - 0.5% Triton X100
Specification
HT1080
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Fixative
Paraformaldehyde
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提交于 Mar 18 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse prostate and mammary epithelial)
Specification
mouse prostate and mammary epithelial
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Fixative
try both PFA and methanol
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提交于 Aug 13 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (MCF10A Breast Cancer Cell Line)
Specification
MCF10A Breast Cancer Cell Line
Permeabilization
No
Fixative
1% Gluteraldehyde
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提交于 May 13 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (breast cell line)
Specification
breast cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Methanol
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提交于 Mar 11 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample
Human Cell (HUVEC)
Specification
HUVEC
Permeabilization
Yes - 0.1% Triton
Fixative
Paraformaldehyde
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提交于 Jan 03 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample
Monkey Cell (COS)
Specification
COS
Permeabilization
Yes - 0.1% Triton
Fixative
Paraformaldehyde
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提交于 Jan 02 2014

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Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (HUVEC)
Specification
HUVEC
Permeabilization
Yes - 0.1% Triton X100
Fixative
Paraformaldehyde
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提交于 Sep 17 2013

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Monkey Cell (cos-7)
Specification
cos-7
Permeabilization
Yes - 0.1% Triton X100
Fixative
Paraformaldehyde
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提交于 Sep 17 2013

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
2% BSA + 5% NGS as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Human Cell (Human umbilical vein endothelial cell)
Specification
Human umbilical vein endothelial cell
Permeabilization
Yes - 0.1% saponin
Fixative
Paraformaldehyde
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提交于 May 28 2013

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