Anti-Pericentrin抗体(ab99341)

概述

  • 产品名称
    Anti-Pericentrin抗体
    参阅全部 Pericentrin 一抗
  • 描述
    兔多克隆抗体to Pericentrin
  • 经测试应用
    适用于: IP, ICC/IF, ICCmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human Pericentrin (NP_006022.3).

  • 阳性对照
    • HeLa whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab99341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
ICC/IF 1/1000 - 1/5000.
ICC 1/1000 - 1/5000.

Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehydefixed cells.

  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
    • 组织特异性
      Expressed in all tissues tested, including placenta, liver, kidney and thymus.
    • 疾病相关
      Microcephalic osteodysplastic primordial dwarfism 2
    • 结构域
      Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
    • 细胞定位
      Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Centrosome Marker antibody
      • Ken antibody
      • Kendrin antibody
      • KIAA0402 antibody
      • MOPD2 antibody
      • PCN antibody
      • PCNT 2 antibody
      • PCNT antibody
      • PCNT B antibody
      • PCNT_HUMAN antibody
      • PCNT1 antibody
      • PCNT2 antibody
      • PCNTB antibody
      • PCTN2 antibody
      • Pericentrin 1 antibody
      • Pericentrin 2 antibody
      • Pericentrin 380 antibody
      • Pericentrin antibody
      • Pericentrin B antibody
      • Pericentrin-B antibody
      • SCKL4 antibody
      see all

    图片

    • Immunocytochemistry/Immunofluorescence analysis of formaldehyde-fixed asynchronous HeLa cells labelling Pericentrin with ab99341 at 1/1000 (1µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
    • Detection of Pericentrin by Western Blot of Immunoprecipitates in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99341 at 3 µg/mg lysate. Anti-Pericentrin antibody ab99342, which recognizes a downstream epitope, was used at 1 µg/ml for subsequent WB detection.

    文献

    ab99341 has not yet been referenced specifically in any publications.

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