The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ICC/IF: 1/100 - 1/500. Formaldehyde-Fixed and acetone-fixed cells.
Permeabilization with Triton-X 100 is recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
组织特异性Expressed in all tissues tested, including placenta, liver, kidney and thymus.
结构域Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
细胞定位Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.