概述

  • 产品名称
    Anti-Pericentrin抗体
    参阅全部 Pericentrin 一抗
  • 描述
    兔多克隆抗体to Pericentrin
  • 经测试应用
    适用于: ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 175 and 225 of Human Pericentrin (NP_006022.3)

  • 阳性对照
    • HeLa Cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab84542 was affinity purified using an epitope specific to Pericentrin immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
  • 应用说明
    ICC/IF: 1/100 - 1/500. Formaldehyde-Fixed and acetone-fixed cells.
    Permeabilization with Triton-X 100 is recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
    • 组织特异性
      Expressed in all tissues tested, including placenta, liver, kidney and thymus.
    • 疾病相关
      Microcephalic osteodysplastic primordial dwarfism 2
    • 结构域
      Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
    • 细胞定位
      Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Centrosome Marker antibody
      • Ken antibody
      • Kendrin antibody
      • KIAA0402 antibody
      • MOPD2 antibody
      • PCN antibody
      • PCNT 2 antibody
      • PCNT antibody
      • PCNT B antibody
      • PCNT_HUMAN antibody
      • PCNT1 antibody
      • PCNT2 antibody
      • PCNTB antibody
      • PCTN2 antibody
      • Pericentrin 1 antibody
      • Pericentrin 2 antibody
      • Pericentrin 380 antibody
      • Pericentrin antibody
      • Pericentrin B antibody
      • Pericentrin-B antibody
      • SCKL4 antibody
      see all

    图片

    • ab84542 at 1/100 dilution staining Pericentrin in HeLa cells by Immunofluorescence, NBF-fixed. Detection: Red-fluorescent Alexa Fluor 594 goat antirabbit IgG.
    • ab84542 at 1/500 dilution staining Pericentrin in asynchronous HeLa cells by Immunofluorescence, NBF-fixed. Detection: Red-fluorescent Alexa Fluor 594 goat antirabbit IgG.
    • ab84542 at 1/500 dilution staining Pericentrin in asynchronous HeLa cells by Immunofluorescence, NBF-fixed. Detection: Red-fluorescent Alexa Fluor 594 goat antirabbit IgG.
    • ab84542 at 1/100 dilution staining Pericentrin in asynchronous HeLa cells by Immunofluorescence, Acetone-fixed. Detection: Red-fluorescent Alexa Fluor 594 goat antirabbit IgG.

    文献

    This product has been referenced in:
    • Bhattacharyya S  et al. Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development. Sci Rep 6:20727 (2016). Read more (PubMed: 26884322) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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