The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100 - 1/500. Formaldehyde-Fixed and acetone-fixed cells.
Permeabilization with Triton-X 100 is recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
Expressed in all tissues tested, including placenta, liver, kidney and thymus.
Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.