Anti-Perforin抗体[CB5.4] (FITC) (ab16075)

概述

  • 产品名称
    Anti-Perforin抗体[CB5.4] (FITC)
    参阅全部 Perforin 一抗
  • 描述
    大鼠单克隆抗体[CB5.4] to Perforin (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用
    适用于: ICC, IHC-P, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 98 - 534 of Mouse Perforin.

  • 表位
    Recognizes mouse perforin, region amino acids 402-534.
  • 阳性对照
    • Mouse T cell clone B6.1.
  • 常规说明
    1 test means: 1µl of a 1mg/ml stock solution were used to stain 200,000 cells in a sample volume of 50µl.

性能

应用

Our Abpromise guarantee covers the use of ab16075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

靶标

  • 功能
    Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • 疾病相关
    Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • 序列相似性
    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • 结构域
    The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • 翻译后修饰
    N-glycosylated.
  • 细胞定位
    Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytolysin antibody
    • FLH2 antibody
    • HPLH2 antibody
    • Lymphocyte pore-forming protein antibody
    • P1 antibody
    • PERF_HUMAN antibody
    • perforin 1 (pore forming protein) antibody
    • Perforin 1 antibody
    • Perforin-1 antibody
    • PFP antibody
    • PGFL antibody
    • PIGF antibody
    • PIGF-2 antibody
    • PLGF antibody
    • Pore forming protein antibody
    • prf1 antibody
    • SHGC-10760 antibody
    see all

文献

This product has been referenced in:
  • Dalton JE  et al. Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells. J Immunol 173:3660-7 (2004). Read more (PubMed: 15356111) »
  • Crider-Pirkle S  et al. Cubilin, a binding partner for galectin-3 in the murine utero-placental complex. J Biol Chem 277:15904-12 (2002). Read more (PubMed: 11856751) »

See all 4 Publications for this product

客户评价及客户问答

Thank you for your enquiry. You should receive 50ul in your vial of ab16075. I hope this helps. Please contact me again if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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