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Our Abpromise guarantee covers the use of ab16074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC||Use a concentration of 5 µg/ml.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab16074 staining Perforin in Mouse lymphoid tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with a zinc-based fixative and blocked with rodent block-M for 20 minutes at 20°C. Samples were incubated with primary antibody (1/50 in TBS + 0.05% Tween20) for 1 hour at 20°C. A HRP-conjugated Goat polyclonal was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"