Anti-Perforin抗体[CB5.4] (ab16074)

概述

  • 产品名称
    Anti-Perforin抗体[CB5.4]
    参阅全部 Perforin 一抗
  • 描述
    大鼠单克隆抗体[CB5.4] to Perforin
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-P, ICC, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 98 - 534 of Mouse Perforin.

  • 表位
    This antibody recognizes mouse perforin, region amino acids 402-534.

性能

应用

Our Abpromise guarantee covers the use of ab16074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • 疾病相关
    Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • 序列相似性
    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • 结构域
    The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • 翻译后修饰
    N-glycosylated.
  • 细胞定位
    Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytolysin antibody
    • FLH2 antibody
    • HPLH2 antibody
    • Lymphocyte pore-forming protein antibody
    • P1 antibody
    • PERF_HUMAN antibody
    • perforin 1 (pore forming protein) antibody
    • Perforin 1 antibody
    • Perforin-1 antibody
    • PFP antibody
    • PGFL antibody
    • PIGF antibody
    • PIGF-2 antibody
    • PLGF antibody
    • Pore forming protein antibody
    • prf1 antibody
    • SHGC-10760 antibody
    see all

Anti-Perforin antibody [CB5.4] 图像

  • ab16074 staining Perforin in Mouse lymphoid tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with a zinc-based fixative and blocked with rodent block-M for 20 minutes at 20°C. Samples were incubated with primary antibody (1/50 in TBS + 0.05% Tween20) for 1 hour at 20°C. A HRP-conjugated Goat polyclonal was used as the secondary antibody.

    See Abreview

Anti-Perforin antibody [CB5.4] (ab16074)参考文献

This product has been referenced in:
  • Ye ZW  et al. Antibody-Dependent Cell-Mediated Cytotoxicity Epitopes on the Hemagglutinin Head Region of Pandemic H1N1 Influenza Virus Play Detrimental Roles in H1N1-Infected Mice. Front Immunol 8:317 (2017). Read more (PubMed: 28377769) »
  • Croci S  et al. Interleukin-15 is required for immunosurveillance and immunoprevention of HER2/neu-driven mammary carcinogenesis. Breast Cancer Res 17:70 (2015). IHC . Read more (PubMed: 25997501) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Cell (uterus, placenta)
Specification
uterus, placenta
Permeabilization
No
Fixative
10% neutral buffered formalin
Username

Dr. Varkha Agrawal

Verified customer

提交于 Jul 03 2013

I can confirm that the Anti-Perforin antibody [CB5.4] (ab16074) has been used with citrate buffer (pH 6) when performing staining of paraffin embedded sections. I would however advise that it may be worthwhile to optimize this for your particular tissu...

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Vielen Dank für Ihre Anfrage.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456 und solltemor...

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Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

Da Ihrem Protokoll bis auf Kleinigkeiten nichts hinzuzufügen ist, muss ich leider davon ausgehen, dass der Antikörper nicht so funktioniert hat, wie er so...

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Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Frageb...

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Thank you for contacting us. Your credit note ID is xxx. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing depar...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lymphoid tissue)
Specification
Lymphoid tissue
Fixative
Zinc
Antigen retrieval step
None
Permeabilization
No
Blocking step
Rodent block-M (other company) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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