Anti-Perforin抗体[B-D48] (Phycoerythrin) (ab47226)


  • 产品名称Anti-Perforin抗体[B-D48] (Phycoerythrin)
    参阅全部 Perforin 一抗
  • 描述
    小鼠单克隆抗体[B-D48] to Perforin (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human perforin.



Our Abpromise guarantee covers the use of ab47226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. Use 10 µl to label 106 cells or 100 µl of whole blood.

ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • 疾病相关Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • 序列相似性Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • 结构域The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytolysin antibody
    • FLH2 antibody
    • HPLH2 antibody
    • Lymphocyte pore-forming protein antibody
    • P1 antibody
    • PERF_HUMAN antibody
    • perforin 1 (pore forming protein) antibody
    • Perforin 1 antibody
    • Perforin-1 antibody
    • PFP antibody
    • PGFL antibody
    • PIGF antibody
    • PIGF-2 antibody
    • PLGF antibody
    • Pore forming protein antibody
    • prf1 antibody
    • SHGC-10760 antibody
    see all

Anti-Perforin antibody [B-D48] (Phycoerythrin) 图像

  • Flow cytometric analysis of Perforin in YT-Indy cell line with ab47226.

Anti-Perforin antibody [B-D48] (Phycoerythrin) (ab47226)参考文献

This product has been referenced in:
  • Ouwendijk WJ  et al. Functional characterization of ocular-derived human alphaherpesvirus cross-reactive CD4 T cells. J Immunol 192:3730-9 (2014). Human . Read more (PubMed: 24623134) »
  • Zaguia F  et al. Cytotoxic NKG2C+ CD4 T cells target oligodendrocytes in multiple sclerosis. J Immunol 190:2510-8 (2013). Flow Cyt ; Human . Read more (PubMed: 23396942) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

I was informed that thelight chain for this antibody is kappa.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


Read More