概述

  • 产品名称Anti-Perforin抗体[B-D48]
    参阅全部 Perforin 一抗
  • 描述
    小鼠单克隆抗体[B-D48] to Perforin
  • 经测试应用适用于: WB, Flow Cyt, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human perforin.

  • 阳性对照
    • IHC-P: Human spleen FFPE tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: PBS
    Sterile-filtered through 0.22 µm and treated to remove endotoxins.
  • Concentration information loading...
  • 纯度Ion Exchange Chromatography
  • 克隆单克隆
  • 克隆编号B-D48
  • 骨髓瘤x63-Ag8.653
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab47225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 20 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • 疾病相关Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • 序列相似性Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • 结构域The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytolysin antibody
    • FLH2 antibody
    • HPLH2 antibody
    • Lymphocyte pore-forming protein antibody
    • P1 antibody
    • PERF_HUMAN antibody
    • perforin 1 (pore forming protein) antibody
    • Perforin 1 antibody
    • Perforin-1 antibody
    • PFP antibody
    • PGFL antibody
    • PIGF antibody
    • PIGF-2 antibody
    • PLGF antibody
    • Pore forming protein antibody
    • prf1 antibody
    • SHGC-10760 antibody
    see all

Anti-Perforin antibody [B-D48] 图像

  • IHC image of Perforin staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47225, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Perforin antibody [B-D48] (ab47225)参考文献

This product has been referenced in:
  • Liu S  et al. Recruitment of Grb2 and SHIP1 by the ITT-like motif of TIGIT suppresses granule polarization and cytotoxicity of NK cells. Cell Death Differ : (2012). Read more (PubMed: 23154388) »
  • Trotta R  et al. The PP2A inhibitor SET regulates granzyme B expression in human natural killer cells. Blood 117:2378-84 (2011). WB ; Human . Read more (PubMed: 21156847) »

See all 3 Publications for this product

Product Wall

You are welcome.

We do not have any data for cross-reactivity with non-primate samples. I tried finding an amino acid sequence for non-primate perforin to align with human perforin, which was the immunogen for ab47225.

The unrevie...

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Thank you for sending the details of your protocol.

I think for PFA-fixed, paraffin-embedded tissue, your protocol is correct. However, our source for this clone has told us that it was only tested in the past for IHC on frozen tissue by col...

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Thank you for bringing this to our attention. To our knowledge, no antigen retrieval should be necessary for histochemistry on frozen tissue sections, or permeabilization assuming your sections are no thicker than 10 microns.

Can you please ...

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Thank you for contacting us. I have doublechecked with the lab and they have confirmed that the antibody ab47225 is provided just in PBS, without any carrier protein or preservative. Format: Phosphate-buffered saline. Sterile-filtered th...

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