概述

  • 产品名称Anti-PER1抗体
    参阅全部 PER1 一抗
  • 描述
    兔多克隆抗体to PER1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Cow, Dog, Pig, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Mouse PER1.

  • 阳性对照
    • This antibody gave a positive signal in Mouse Testis tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab99253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 136 kDa).

靶标

  • 功能Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
    NPAS2-BMAL1
    BMAL2-induced transactivation.
  • 组织特异性Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney.
  • 序列相似性Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 翻译后修饰Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated.
  • 细胞定位Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Circadian clock protein PERIOD 1 antibody
    • Circadian clock protein PERIOD1 antibody
    • Circadian pacemaker protein Rigui antibody
    • hPER 1 antibody
    • hPER antibody
    • hPER1 antibody
    • KIAA0482 antibody
    • MGC88021 antibody
    • PER 1 antibody
    • PER antibody
    • PER1 antibody
    • PER1 protein antibody
    • PER1_HUMAN antibody
    • Period 1 antibody
    • Period circadian protein homolog 1 antibody
    • Period drosophila homolog of antibody
    • Period homolog 1 antibody
    • Period1 antibody
    • RIGUI antibody
    see all

Anti-PER1 antibody 图像

  • Anti-PER1 antibody (ab99253) at 1 µg/ml + Testis (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 136 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-PER1 antibody (ab99253)参考文献

ab99253 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99253.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"