Anti-Pepsinogen I抗体[7G3] (ab50123)

概述

  • 产品名称Anti-Pepsinogen I抗体[7G3]
    参阅全部 Pepsinogen I 一抗
  • 描述
    小鼠单克隆抗体[7G3] to Pepsinogen I
  • 经测试应用适用于: IHC-P, ELISA, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.15M Sodium chloride, 15mM PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号7G3
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use a concentration of 1 - 10 µg/ml. Can be paired for ELISA with Mouse monoclonal [2F5] to Pepsinogen I (ab50607).
Sandwich ELISA Use at an assay dependent concentration.

靶标

  • 功能Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
  • 序列相似性Belongs to the peptidase A1 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PEPA3_HUMAN antibody
    • Pepsin A-3 antibody
    • Pepsinogen 3 group I antibody
    • PEPSINOGEN I--SECOND LOCUS antibody
    • Pepsinogen-3 antibody
    • PGA3 antibody
    see all

Anti-Pepsinogen I antibody [7G3] 图像

  • IHC image of ab50123 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50123, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Pepsinogen I antibody [7G3] (ab50123)参考文献

ab50123 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Specification Intestine
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative Paraformaldehyde
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Verified customer

提交于 Aug 03 2015

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Aug 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Stomach)
Specification Stomach
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Apr 12 2012

Thank you for your enquiry. I am sorry it has taken some time to confirm this information. I can confirm that for sandwich ELISA the following have been used for testing: Capture antibody : ab50607 Anti-Pepsinogen I antibody 2F5 Detection antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"