The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).
功能Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal.
序列相似性Belongs to the VPS10-related sortilin family. Contains 10 BNR repeats.
结构域The lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal.
细胞定位Golgi apparatus > trans-Golgi network membrane. Prevacuolar compartment membrane. Cycles between the Golgi apparatus and the prevacuolar compartments.