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Our Abpromise guarantee covers the use of ab13812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/500 - 1/1000. Predicted molecular weight: 46 kDa.|
ab13812 staining Pellino 1 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/1000) was used as the secondary antibody..
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