概述

  • 产品名称
    Anti-Pellino 1抗体
    参阅全部 Pellino 1 一抗
  • 描述
    兔多克隆抗体to Pellino 1
  • 经测试应用
    适用于: ICC, ICC/IF, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    SQSNSDTQSV QSTIS

    conjugated to KLH, corresponding to amino acids 121-135 of Human Pellino 1

  • 阳性对照
    • Human Ramos cell lysate, mouse Raw cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab13812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.

靶标

图片

  • ab13812 staining Pellino 1 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/1000) was used as the secondary antibody..

    See Abreview

  • All lanes : Anti-Pellino 1 antibody (ab13812) at 1/1000 dilution

    Lane 1 : Lysates prepared from human Ramos cell line
    Lane 2 : Lysates prepared from mouse Raw cell line


    Predicted band size : 46 kDa
    Western blot analysis of Pellino1 in human Ramos cell lysate (A) and mouse Raw cell lysate (B) using ab13812 at 1/1000

文献

This product has been referenced in:
  • Ma X  et al. Interaction of the oncogenic miR-21 microRNA and the p53 tumor suppressor pathway. Carcinogenesis 34:1216-23 (2013). Mouse . Read more (PubMed: 23385064) »
  • Kinsella S  et al. Bid Promotes K63-Linked Polyubiquitination of Tumor Necrosis Factor Receptor Associated Factor 6 (TRAF6) and Sensitizes to Mutant SOD1-Induced Proinflammatory Signaling in Microglia. eNeuro 3:N/A (0). Read more (PubMed: 27257617) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW264.7)
Specification
RAW264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton + 2% BSA in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Platelets)
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Platelets
Username

Abcam user community

Verified customer

提交于 Dec 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PMN)
Specification
PMN
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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