概述

  • 产品名称Anti-PEG10抗体
    参阅全部 PEG10 一抗
  • 描述
    小鼠多克隆抗体to PEG10
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human), PEG10 (NP_001035242.1, 325 a.a)

  • 阳性对照
    • Human stomach tissue lysate, PEG10 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab68780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects bands of approximately 42 and 100 kDa (predicted molecular weight for isoform RF1: 37 kDa. predicted molecular weight for isoform RF1/RF2:80 kDa ).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.
    • 组织特异性Expressed in the cytotrophoblast layer but not in the overlying syncytiotrophoblast of the placenta. Expressed in prostate and breast carcinomas but not in normal breast and prostate epithelial cells. Expressed in the HepG2 cell line (at protein level). Expressed in brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph node, skeletal muscle, adipose tissue and placenta. Expressed in pancreatic and hepatocellular carcinomas (HCC).
    • 序列相似性Contains 1 CCHC-type zinc finger.
    • 发展阶段Expressed in placenta during the first trimester of gestation (at protein level). In placenta, down-regulated at early hypoxic phase, and highly activated at 11-12 week of gestation.
    • 翻译后修饰Isoform RF1/RF2 undergoes proteolytic cleavage.
    • 细胞定位Nucleus. Cytoplasm. Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the HepG2 cell line. Colocalized with ALK1.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AA407948 antibody
      • Edr antibody
      • Embryonal carcinoma differentiation regulated antibody
      • Embryonal carcinoma differentiation-regulated protein antibody
      • HB 1 antibody
      • HB1 antibody
      • KIAA1051 antibody
      • Mammalian retrotransposon-derived protein 2 antibody
      • Mar2 antibody
      • Mart2 antibody
      • MEF3 like 1 antibody
      • MEF3-like protein 1 antibody
      • MEF3L antibody
      • MEF3L1 antibody
      • MyEF 3 antibody
      • Myelin expression factor 3-like protein 1 antibody
      • Paternally expressed 10 antibody
      • Paternally expressed gene 10 ORF1 antibody
      • Paternally expressed gene 10 protein antibody
      • Peg10 antibody
      • PEG10 protein antibody
      • PEG10_HUMAN antibody
      • Putative uncharacterized protein PEG10 antibody
      • Retrotransposon gag domain containing 3 antibody
      • Retrotransposon gag domain-containing protein 3 antibody
      • Retrotransposon-derived gag-like polyprotein antibody
      • Retrotransposon-derived protein PEG10 antibody
      • RGAG3 antibody
      • Ty3/Gypsy-like protein antibody
      see all

    Anti-PEG10 antibody 图像

    • Anti-PEG10 antibody (ab68780) at 1/500 dilution + Human stomach tissue lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 37 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-PEG10 antibody (ab68780) at 1/500 dilution

      Lane 1 : PEG10 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 37 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    Anti-PEG10 antibody (ab68780)参考文献

    ab68780 has not yet been referenced specifically in any publications.

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