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Abcam's PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate viruses without ultra-centrifugation. The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer. The kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples. Viruses can be concentrated over 100 fold. An optimized Virus Re-suspension Solution is provided to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.
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Viruses are usually produced at a low titer. They often need to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate viruses and remove impurities.
|PEG Solution||1 x 125ml|
|Virus Re-suspension Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab102538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Concentration of baculovirus: Low titer baculovirus (10 ml) expressing human Granzyme B was precipitated following the kit protocol and the precipitate was suspended in 1 ml of Virus Re-suspension Solution. Both low titer and precipitated baculovirus was subsequently used to infect SF9 Insect cells. The activity of recombinant Granzyme B secreted into the culture medium by low titer and precipitated baculovirus infected insect cells was monitored using ab157403 - Granzyme B Activity Assay Kit (Fluorometric).