Mouse, Rat, Cow, Monkey
Recombinant protein fragment with a sequence corresponding to a region within amino acids 77 and 265 of Human PEF1 (NP_036524).
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji cell lines. HeLa cells for IF.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 30 kDa.
||1/100 - 1/200.
Contains 5 EF-hand domains.
Cytoplasm. Membrane. Membrane-associated in the presence of C.
Information by UniProt
- ABP32 antibody
- PEF antibody
- PEF protein with a long N terminal hydrophobic domain antibody
Western blot - Anti-PEF1 antibody (ab96132)
Anti-PEF1 antibody (ab96132) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PEF1 antibody (ab96132)
ab96132, at 1/200 staining HeLa cells by Immunofluorescence. Lower image merged with DNA probe.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"