概述

  • 产品名称Anti-PEDF抗体
    参阅全部 PEDF 一抗
  • 描述
    兔多克隆抗体to PEDF
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PEDF.

    (Peptide available as ab74438.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Tissue lysates: Plasma Total Protein Lysate, Kidney, Ovary (data not shown), Skeletal Muscle (data not shown).

性能

应用

Our Abpromise guarantee covers the use of ab74439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
    • 组织特异性Retinal pigment epithelial cells and blood plasma.
    • 序列相似性Belongs to the serpin family.
    • 发展阶段Expressed in quiescent cells.
    • 结构域The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
    • 翻译后修饰The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
      N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • 细胞定位Secreted. Melanosome. Enriched in stage I melanosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cell proliferation-inducing gene 35 protein antibody
      • EPC 1 antibody
      • EPC-1 antibody
      • EPC1 antibody
      • OI12 antibody
      • OI6 antibody
      • PEDF antibody
      • PEDF_HUMAN antibody
      • PIG 35 antibody
      • PIG35 antibody
      • Pigment epithelium derived factor antibody
      • Pigment epithelium-derived factor antibody
      • Proliferation inducing protein 35 antibody
      • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
      • Serpin F1 antibody
      • Serpin family F member 1 antibody
      • Serpin peptidase inhibitor clade F member 1 antibody
      • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
      • SERPINF 1 antibody
      • Serpinf1 antibody
      see all

    Anti-PEDF antibody 图像

    • All lanes : Anti-PEDF antibody (ab74439) at 1 µg/ml

      Lane 1 : Human Plasma Total Protein Lysate
      Lane 2 : Human kidney tissue lysate - total protein (ab30203)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
      Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds

    Anti-PEDF antibody (ab74439)参考文献

    ab74439 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab74439.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"