Anti-PEDF抗体(ab14993)
Key features and details
- Rabbit polyclonal to PEDF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PEDF抗体
参阅全部 PEDF 一抗 -
描述
兔多克隆抗体to PEDF -
宿主
Rabbit -
特异性
Recognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein (His-tag) corresponding to Human PEDF. Expressed in baby hamster kidney cells.
Database link: P36955 -
阳性对照
- WB: RIPA lysates from Y79 cells.
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常规说明
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14993于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
靶标
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功能
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
组织特异性
Retinal pigment epithelial cells and blood plasma. -
序列相似性
Belongs to the serpin family. -
发展阶段
Expressed in quiescent cells. -
结构域
The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. -
翻译后修饰
The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
细胞定位
Secreted. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 5176 Human
- Omim: 172860 Human
- SwissProt: P36955 Human
- Unigene: 532768 Human
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别名
- Cell proliferation-inducing gene 35 protein antibody
- EPC 1 antibody
- EPC-1 antibody
see all
图片
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Lanes 1-4: Cartilage lysates from 4 osteoarthritic knee replacement donors (100 mg (wet weight) powdered cartilage/ 1 ml RIPA buffer, extracted for 24 hrs at 4°C)
Lane C: Positive control (overexpressing cell line)
Anticipated band at approx 50kDa detected. Specificity of antibody allowed relatively long exposure and quite high concentrations of primary and secondary antibodies to be used with negligible background. Good results were also obtained with unreduced samples.
The additional approx 250kDa band is likely to be due to PEDF crosslinked or bound to high molecular weight matrix proteins (collagen, aggrecan) which are present in cartilage lysates. Reduction using beta mercaptoethanol reduced some, but not all high MW staining and increased the intensity of the 50kDa band. -
Anti-PEDF antibody (ab14993) at 1 µg/ml + Y79 (Human retinoblastoma cell line) cell lysate in RIPA buffer at 40 µg
Secondary
IgG (HRP) goat anti-rabbit
Predicted band size: 50 kDaChemiluminescence detection system.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab14993 被引用在 9 文献中.
- Sun X et al. Improvement of the bladder perfusion curative effect through tight junction protein degradation induced by magnetic temperature-sensitive hydrogels. Front Bioeng Biotechnol 10:958072 (2022). PubMed: 35992356
- Li B et al. lncRNA GABPB1 intronic transcript 1 upregulates pigment epithelium-derived factor via miR-93 to suppress cell proliferation in hepatocellular carcinoma. Oncol Lett 21:260 (2021). PubMed: 33664823
- Zhang X et al. Pigment epithelium-derived factor facilitates NLRP3 inflammasome activation through downregulating cytidine monophosphate kinase 2: A potential treatment strategy for missed abortion. Int J Mol Med 45:1436-1446 (2020). PubMed: 32323732
- Qian W et al. Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17:6515-6525 (2018). WB . PubMed: 29532868
- Han Z et al. Retinal angiogenesis in the Ins2(Akita) mouse model of diabetic retinopathy. Invest Ophthalmol Vis Sci 54:574-84 (2013). WB . PubMed: 23221078
- Allen CL et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One 8:e78441 (2013). ICC/IF . PubMed: 24205233
- Tripathy D et al. Age-related decrease in cerebrovascular-derived neuroprotective proteins: effect of acetaminophen. Microvasc Res 84:278-85 (2012). ELISA ; Rat . PubMed: 22944728
- Sanchez A et al. Pigment epithelium-derived factor (PEDF) protects cortical neurons in vitro from oxidant injury by activation of extracellular signal-regulated kinase (ERK) 1/2 and induction of Bcl-2. Neurosci Res 72:1-8 (2012). ELISA . PubMed: 21946416
- Simper D et al. Comparative proteomics profiling reveals role of smooth muscle progenitors in extracellular matrix production. Arterioscler Thromb Vasc Biol 30:1325-32 (2010). WB ; Human . PubMed: 20431068