概述

  • 产品名称Anti-PEDF抗体[1C4]
    参阅全部 PEDF 一抗
  • 描述
    小鼠单克隆抗体[1C4] to PEDF
  • 经测试应用适用于: ELISA, Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 19-418 of Human PEDF purified from E. coli.

  • 阳性对照
    • A549 lysate; Human Kidney and Placenta tissues.

性能

应用

Our Abpromise guarantee covers the use of ab115489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 46 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • 组织特异性Retinal pigment epithelial cells and blood plasma.
  • 序列相似性Belongs to the serpin family.
  • 发展阶段Expressed in quiescent cells.
  • 结构域The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
  • 翻译后修饰The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位Secreted. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation-inducing gene 35 protein antibody
    • EPC 1 antibody
    • EPC-1 antibody
    • EPC1 antibody
    • OI12 antibody
    • OI6 antibody
    • PEDF antibody
    • PEDF_HUMAN antibody
    • PIG 35 antibody
    • PIG35 antibody
    • Pigment epithelium derived factor antibody
    • Pigment epithelium-derived factor antibody
    • Proliferation inducing protein 35 antibody
    • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • Serpin F1 antibody
    • Serpin family F member 1 antibody
    • Serpin peptidase inhibitor clade F member 1 antibody
    • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • SERPINF 1 antibody
    • Serpinf1 antibody
    see all

Anti-PEDF antibody [1C4] 图像

  • Overlay histogram showing K562 cells stained with ab115489 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115489, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-PEDF antibody [1C4] (ab115489) at 1/1000 dilution + A549 cell lysate
    Developed using the ECL technique

    Predicted band size : 46 kDa
  • ab115489 10 ug/ml, staining PEDF in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115489 10 ug/ml, staining PEDF in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-PEDF antibody [1C4] (ab115489)参考文献

This product has been referenced in:
  • Qian J  et al. Downregulation of HIF-1a inhibits the proliferation and invasion of non-small cell lung cancer NCI-H157 cells. Oncol Lett 11:1738-1744 (2016). Read more (PubMed: 26998070) »

See 1 Publication for this product

Product Wall

Gracias por contactarnos.

Efectivamente este anticuerpo ha sido validado en nuestro laboratorio en ensayos de ELISA indirecto. Cubriendo la placa con el antígeno, incubando con el primario, y detectando posteriormente con un secundario...

Read More




I am very pleased to hear you would like to accept our offer and test ab14993, ab30788 ,ab64053 and ab115489 in sELISA. These codes will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please s...

Read More

Thank you very much for your interest in PEDF and Somatostatin antibodies.

To our knowledge,none of our antibodies have been tested in sELISA. However, I have picked out four antibodies, which I found most suitable for this purpose:
Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"